Detail publikace

Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment

SZTALMACHOVÁ, M. GUMULEC, J. RAUDENSKÁ, M. POLANSKÁ, H. HOLUBOVÁ, M. BALVAN, J. HUDCOVÁ, K. KNOPFOVÁ, L. KÍZEK, R. ADAM, V. BABULA, P. MASAŘÍK, M.

Originální název

Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Breast cancer patients negative for the nuclear oestrogen receptor alpha have a particularly poor prognosis. Therefore, the 4T1 cell line (considered as a triple-negative model) was chosen to induce malignancy in mice. The aim of the present study was to assess if zinc ions, provided in excess, may significantly modify the process of mammary oncogenesis. Zn(II) ions were chosen because of their documented antitumour effects. Zn(II) is also known to induce the expression of metallothioneins (MT) and glutathion (GSH). A total dose of zinc sulphate per one gram of mouse weight used in the experiment was 0.15 mg. We studied the expression of MT1 MT2, TP53 and MTF-1genes and also examined the effect of the tumour on antioxidant capacity. Tumour-free mice had significantly higher expression levels of the studied genes (P<0.003). Significant differences were also revealed in the gene expression between the tissues (P<0.001). The highest expression levels were observed in the liver. As compared to brain, lung and liver, significantly lower concentrations of MT protein were found in the primary tumour; an inverse trend was observed in the concentration of Zinc(II). In non-tumour mice, the amount of hepatic hydrosulphuryl groups significantly increased by the exposure to Zn(II), but the animals with tumour induction showed no similar trend. The primary tumour size of zinc-treated animals was 20% smaller (P=0.002); however, no significant effect on metastasis progression due to the zinc treatment was discovered. In conclusion, Zn(II) itself may mute the growth of primary breast tumours especially at their early stages.

Klíčová slova

breast cancer; zinc treatment; tumour size; metallothionein; glutathione

Autoři

SZTALMACHOVÁ, M.; GUMULEC, J.; RAUDENSKÁ, M.; POLANSKÁ, H.; HOLUBOVÁ, M.; BALVAN, J.; HUDCOVÁ, K.; KNOPFOVÁ, L.; KÍZEK, R.; ADAM, V.; BABULA, P.; MASAŘÍK, M.

Rok RIV

2015

Vydáno

1. 4. 2015

ISSN

1019-6439

Periodikum

INTERNATIONAL JOURNAL OF ONCOLOGY

Ročník

46

Číslo

4

Stát

Řecká republika

Strany od

1810

Strany do

1818

Strany počet

9

BibTex

@article{BUT123337,
  author="Markéta {Sztalmachová} and Vojtěch {Adam} and Petr {Babula} and Michal {Masařík} and Jaromír {Gumulec} and Martina {Raudenská} and Hana {Polanská} and Monika {Kratochvílová} and René {Kizek} and Jan {Balvan} and Kristýna {Hudcová} and Lucia {Knopfová}",
  title="Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment",
  journal="INTERNATIONAL JOURNAL OF ONCOLOGY",
  year="2015",
  volume="46",
  number="4",
  pages="1810--1818",
  doi="10.3892/ijo.2015.2883",
  issn="1019-6439"
}