Detail publikace

Characterization of multi-walled carbon nanotubes double-functionalization with cytostatic drug etoposide and phosphorothioate oligodeoxynucleotides

HEGER, Z. MOULICK, A. NGUYEN, H. KREMPLOVÁ, M. KOPEL, P. HYNEK, D. ECKSCHLAGER, T. STIBOROVÁ, M. ZÍTKA, O. ADAM, V. KÍZEK, R.

Originální název

Characterization of multi-walled carbon nanotubes double-functionalization with cytostatic drug etoposide and phosphorothioate oligodeoxynucleotides

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Carbon nanomaterials possess unique structural and physicochemical properties, and thus they are broadly utilized as carriers for a variety of therapeutic agents in nanomedicinal applications. Herein we present an electrochemical characterization of binding capability of acidic oxidized multi-walled carbon nanotubes (oMWCNTs) modified with poly(ethylene glycol) towards common cytostatic drug etoposide (or VP-16). The comparison of willingness of oMWCNTs and MWCNTs-PEG to form complexes with etoposide revealed significantly higher capacity in PEGylated variant of carbon nanotubes (the 15 mM etoposide loading capacity was approximately 46.4% in 2 mg of MWCNT-PEG or 28.1% in equal amount of oMWCNT). To obtain a multifunctional nanotransporter we employed the phosphorothioate oligodeoxynucleotide (PODN), which could further extend the possible biological effects of MWCNT-PEG-Etoposide complex. By using square wave voltammetry, the binding capacity of 2 mg of MWCNT-PEG-Etoposide (15 mM) towards PODNs was determined to be 4.5 mu M. Such characterized multifunctional complex can be further employed for biological testing on chemoresistant tumors, such as non-small-cell lung cancer, to enhance the treatment efficiency.

Klíčová slova

Antisense oligodeoxynucleotides; Carbon nanomaterials; Electrochemistry; Nanotechnology; Poly(ethylene) glycol

Autoři

HEGER, Z.; MOULICK, A.; NGUYEN, H.; KREMPLOVÁ, M.; KOPEL, P.; HYNEK, D.; ECKSCHLAGER, T.; STIBOROVÁ, M.; ZÍTKA, O.; ADAM, V.; KÍZEK, R.

Rok RIV

2015

Vydáno

1. 9. 2015

Nakladatel

ESG

ISSN

1452-3981

Periodikum

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Ročník

10

Číslo

9

Stát

Srbská republika

Strany od

7707

Strany do

7719

Strany počet

13

URL

http://www.electrochemsci.org/papers/vol10/100907707.pdf

Plný text v Digitální knihovně

http://hdl.handle.net/11012/201457

BibTex

@article{BUT123363,
  author="Zbyněk {Heger} and Amitava {Moulick} and Hoai Viet {Nguyen} and Monika {Kremplová} and Pavel {Kopel} and David {Hynek} and Tomáš {Eckschlager} and Marie {Stiborová} and Ondřej {Zítka} and Vojtěch {Adam} and René {Kízek}",
  title="Characterization of multi-walled carbon nanotubes double-functionalization with cytostatic drug etoposide and phosphorothioate oligodeoxynucleotides",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2015",
  volume="10",
  number="9",
  pages="7707--7719",
  issn="1452-3981",
  url="http://www.electrochemsci.org/papers/vol10/100907707.pdf"
}