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BURDÍKOVÁ, J. MRAVEC, F. PEKAŘ, M.
Originální název
Steady-State Fluorescence Spectroscopy Techniques in Determination of Phospholipids Vesicles Properties
Typ
abstrakt
Jazyk
angličtina
Originální abstrakt
Steady-state fluorescence techniques represent a powerful tool for the determination of great variety of phospholipids vesicles (liposome) properties. Phase transition temperature is one of the most important characteristics of liposomes. It is commonly determined by means of calorimetric technics. It can be also obtained by analyzing of laurdan emission spectra at different temperatures. The degree of a lipid acyl chain order in liposomes and a membrane fluidity can by studied by means of fluorescence anisotropy, which measures depolarization of fluorophores excited by polarized light, where the depolarization depends on the membrane fluidity. Diphenylhexatriene is a suitable fluorescence probe for the anisotropy measurements. The aggregation behavior of lipid molecules can be studied using fluorescence probes such as pyrene or perylene. These fluorescence probes are sensitive to the polarity of the local environment and indicate changes in lipid aggregates character. Fluorescent lipophilic tracers such a DiO or DiA are usually applied in a staining of the cell surface, but they can be easily used also in aggregates formation study.
Klíčová slova
steady-state fluorescence spectroscopy, lipid, liposome, pyrene, laurdan, fluorescent lipophilic tracers, fluorescence anisotropy, DPH
Autoři
BURDÍKOVÁ, J.; MRAVEC, F.; PEKAŘ, M.
Vydáno
3. 12. 2015
Nakladatel
Vysoké učení technické v Brně, Fakulta chemická
Místo
Brno
ISBN
978-80-214-5290-9
Kniha
Sborník abstraktů: Studentská konference Chemie je život
Číslo edice
první
Strany od
61
Strany do
Strany počet
1
BibTex
@misc{BUT124991, author="Jana {Burdíková} and Filip {Mravec} and Miloslav {Pekař}", title="Steady-State Fluorescence Spectroscopy Techniques in Determination of Phospholipids Vesicles Properties", booktitle="Sborník abstraktů: Studentská konference Chemie je život", year="2015", edition="první", pages="61--61", publisher="Vysoké učení technické v Brně, Fakulta chemická", address="Brno", isbn="978-80-214-5290-9", note="abstract" }