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Detail publikace
DOSTÁLOVÁ, S. ČERNÁ, T. HYNEK, D. KOUDELKOVÁ, Z. VACULOVIČ, T. KOPEL, P. HRABĚTA, J. HEGER, Z. VACULOVIČOVÁ, M. ECKSCHLAGER, T. ADAM, V.
Originální název
Site-Directed Conjugation of Antibodies to Apoferritin Nanocarrier for Targeted Drug Delivery to Prostate Cancer Cells
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Herein, we describe a novel approach for targeting of ubiquitous protein apoferritin (APO)-encapsulating doxorubicin (DOX) to prostate cancer using antibodies against prostatespecific membrane antigen (PSMA). The conjugation of anti-PSMA antibodies and APO was carried out using HWRGWVC heptapeptide, providing their site-directed orientation. The prostatecancer- targeted and nontargeted nanocarriers were tested using LNCaP and HUVEC cell lines. A total of 90% of LNCaP cells died after treatment with DOX (0.25 μM) or DOX in nontargeted and prostate-cancer-targeted APO, proving that the encapsulated DOX toxicity for LNCaP cells remained the same. Free DOX showed higher toxicity for nonmalignant cells, whereas the toxicity was lower after treatment with the same dosage of APO-encapsulated DOX (APODOX) and even more in prostate-cancer-targeted APODOX. Hemolytic assay revealed exceptional hemocompatibility of the entire nanocarrier. The APO encapsulation mechanism ensures applicability using a wide variety of chemotherapeutic drugs, and the presented surface modification enables targeting to various tumors.
Klíčová slova
antibodies, apoferritin, doxorubicin, nanomedicine, targeted drug delivery
Autoři
DOSTÁLOVÁ, S.; ČERNÁ, T.; HYNEK, D.; KOUDELKOVÁ, Z.; VACULOVIČ, T.; KOPEL, P.; HRABĚTA, J.; HEGER, Z.; VACULOVIČOVÁ, M.; ECKSCHLAGER, T.; ADAM, V.
Vydáno
15. 6. 2016
ISSN
1944-8252
Periodikum
ACS applied materials & interfaces
Ročník
8
Číslo
23
Stát
Spojené státy americké
Strany od
14430
Strany do
14441
Strany počet
12
BibTex
@article{BUT126821, author="Simona {Dostálová} and Tereza {Černá} and David {Hynek} and Zuzana {Koudelková} and Tomáš {Vaculovič} and Pavel {Kopel} and Jan {Hraběta} and Zbyněk {Heger} and Markéta {Vaculovičová} and Tomáš {Eckschlager} and Vojtěch {Adam}", title="Site-Directed Conjugation of Antibodies to Apoferritin Nanocarrier for Targeted Drug Delivery to Prostate Cancer Cells", journal="ACS applied materials & interfaces", year="2016", volume="8", number="23", pages="14430--14441", doi="10.1021/acsami.6b04286", issn="1944-8252" }