Detail publikace

Site-Directed Conjugation of Antibodies to Apoferritin Nanocarrier for Targeted Drug Delivery to Prostate Cancer Cells

DOSTÁLOVÁ, S. ČERNÁ, T. HYNEK, D. KOUDELKOVÁ, Z. VACULOVIČ, T. KOPEL, P. HRABĚTA, J. HEGER, Z. VACULOVIČOVÁ, M. ECKSCHLAGER, T. ADAM, V.

Originální název

Site-Directed Conjugation of Antibodies to Apoferritin Nanocarrier for Targeted Drug Delivery to Prostate Cancer Cells

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Herein, we describe a novel approach for targeting of ubiquitous protein apoferritin (APO)-encapsulating doxorubicin (DOX) to prostate cancer using antibodies against prostatespecific membrane antigen (PSMA). The conjugation of anti-PSMA antibodies and APO was carried out using HWRGWVC heptapeptide, providing their site-directed orientation. The prostatecancer- targeted and nontargeted nanocarriers were tested using LNCaP and HUVEC cell lines. A total of 90% of LNCaP cells died after treatment with DOX (0.25 μM) or DOX in nontargeted and prostate-cancer-targeted APO, proving that the encapsulated DOX toxicity for LNCaP cells remained the same. Free DOX showed higher toxicity for nonmalignant cells, whereas the toxicity was lower after treatment with the same dosage of APO-encapsulated DOX (APODOX) and even more in prostate-cancer-targeted APODOX. Hemolytic assay revealed exceptional hemocompatibility of the entire nanocarrier. The APO encapsulation mechanism ensures applicability using a wide variety of chemotherapeutic drugs, and the presented surface modification enables targeting to various tumors.

Klíčová slova

antibodies, apoferritin, doxorubicin, nanomedicine, targeted drug delivery

Autoři

DOSTÁLOVÁ, S.; ČERNÁ, T.; HYNEK, D.; KOUDELKOVÁ, Z.; VACULOVIČ, T.; KOPEL, P.; HRABĚTA, J.; HEGER, Z.; VACULOVIČOVÁ, M.; ECKSCHLAGER, T.; ADAM, V.

Vydáno

15. 6. 2016

ISSN

1944-8252

Periodikum

ACS applied materials & interfaces

Ročník

8

Číslo

23

Stát

Spojené státy americké

Strany od

14430

Strany do

14441

Strany počet

12

BibTex

@article{BUT126821,
  author="Simona {Dostálová} and Tereza {Černá} and David {Hynek} and Zuzana {Koudelková} and Tomáš {Vaculovič} and Pavel {Kopel} and Jan {Hraběta} and Zbyněk {Heger} and Markéta {Vaculovičová} and Tomáš {Eckschlager} and Vojtěch {Adam}",
  title="Site-Directed Conjugation of Antibodies to Apoferritin Nanocarrier for Targeted Drug Delivery to Prostate Cancer Cells",
  journal="ACS applied materials & interfaces",
  year="2016",
  volume="8",
  number="23",
  pages="14430--14441",
  doi="10.1021/acsami.6b04286",
  issn="1944-8252"
}