Detail publikace

Bioconjugation of peptides using advanced nanomaterials to examine their interactions in 3D printed flow-through device

MICHÁLEK, P. RICHTERA, L. KREJČOVÁ, L. NEJDL, L. KENŠOVÁ, R. ZÍTKA, J. KOPEL, P. HEGER, Z. ADAM, V. KIZEK, R.

Originální název

Bioconjugation of peptides using advanced nanomaterials to examine their interactions in 3D printed flow-through device

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Peptide–peptide interactions are crucial in the living cell as they lead to the formation of the numerous types of complexes. In this study, synthetic peptides containing 11 of cysteines (-domain of metallothionein (MT)) and sialic acid binding region (130-loop of hemagglutinin (HA)) were employed. The aim of the experiment was studying the interactions between MT and HA-derived peptides. For this purpose, fragments were tagged with cysteines at C-terminal part to serve as ligand sites for PbS and CuS quantum dots (QDs), and therefore these conjugates can be traced and quantified during wide spectrum of methods. As a platform for interaction, -Fe2O3 paramagnetic particles modified with tetraethyl orthosilicate and (3-aminopropyl)triethoxysilane (hydrodynamic diameter 30– 40 nm) were utilized and MT/HA interactions were examined using multi-instrumental approach including electrochemistry, electrophoretic methods, and MALDI-TOF/TOF mass spectrometry. It was found that peptides enter mutual creation of complexes, which are based on some of nonbonded interactions. The higher willingness to interact was observed in MT-derived peptides toward immobilized HA. Finally, we designed and manufactured flow-through electrochemical 3D printed device (reservoir volume 150 L) and utilized it for automated analysis of the HA/MT metal labels. Under the optimal conditions, (deposition time and flow rate 80 s and 1.6 mL/min for CuS and 120 s and 1.6 mL/min PbS, respectively), the results of peptide-conjugated QDs were comparable with atomic absorption spectrometry.

Klíčová slova

3D printing / Advanced materials / Influenza / Lab-On-a-Chip / Magnetic particle

Autoři

MICHÁLEK, P.; RICHTERA, L.; KREJČOVÁ, L.; NEJDL, L.; KENŠOVÁ, R.; ZÍTKA, J.; KOPEL, P.; HEGER, Z.; ADAM, V.; KIZEK, R.

Vydáno

1. 2. 2016

ISSN

0173-0835

Periodikum

Electrophoresis

Ročník

37

Číslo

3

Stát

Spolková republika Německo

Strany od

444

Strany do

454

Strany počet

11

BibTex

@article{BUT126826,
  author="Petr {Michálek} and Lukáš {Richtera} and Ludmila {Krejčová} and Lukáš {Nejdl} and Renáta {Kenšová} and Jan {Zítka} and Pavel {Kopel} and Zbyněk {Heger} and Vojtěch {Adam} and René {Kizek}",
  title="Bioconjugation of peptides using advanced nanomaterials to examine their interactions in 3D printed flow-through device",
  journal="Electrophoresis",
  year="2016",
  volume="37",
  number="3",
  pages="444--454",
  doi="10.1002/elps.201500345",
  issn="0173-0835"
}