Detail publikace

3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

HEGER, Z. ZÍTKA, J. NEJDL, L. MOULICK, A. MILOSAVLJEVIĆ, V. KOPEL, P. ZÁVODSKÝ, O. KAPUŠ, J. LENŽA, L. ŘEZKA, M. ADAM, V. KIZEK, R.

Originální název

3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

We present a utilization of carbon quantum dots (CQDs), passivated with polyethylene glycol as a fluorescent recognition probe for DNA damage. Synthesized CQDs were characterized in detail, using optical and electrochemical methods. Further, fluorescent behavior of CQDs was monitored in the presence of genomic DNA, isolated from Staphylococcus aureus. In laboratory conditions, after 30 min of exposure to UV irradiation (k = 254 nm), the DNA/CQDs complex significantly increased its fluorescence. Further, stratospheric probe was suggested and crafted by using technology of 3D printing (acrylonitrile–butadiene–styrene as a material). CQDs were exploited to evaluate the DNA damage in stratospheric conditions (up to 20,000 m) by determination of fluorescence increase (kexc = 245 nm, kem = 400 nm), together with other parameters (temperature, humidity, altitude, pressure, UV intensity, and X-ray irradiation). The obtained data showed that the sensor utilizing the DNA/ CQDs was able to identify the DNA damage, together with external conditions. It was shown the proposed concept is able to operate at temperatures lower than -70 C. The proposed protocol may by applicable as a biosensor for long-term space missions, like international space station, missions to the Moon or Mars.

Klíčová slova

Carbon nanomaterials, Near-space conditions, Radiation, Ultraviolet spectrum

Autoři

HEGER, Z.; ZÍTKA, J.; NEJDL, L.; MOULICK, A.; MILOSAVLJEVIĆ, V.; KOPEL, P.; ZÁVODSKÝ, O.; KAPUŠ, J.; LENŽA, L.; ŘEZKA, M.; ADAM, V.; KIZEK, R.

Vydáno

1. 5. 2016

ISSN

1434-4475

Periodikum

Monatshefte für Chemie

Ročník

147

Číslo

5

Stát

Rakouská republika

Strany od

873

Strany do

880

Strany počet

8

BibTex

@article{BUT127766,
  author="Zbyněk {Heger} and Jan {Zítka} and Lukáš {Nejdl} and Amitava {Moulick} and Vedran {Milosavljević} and Pavel {Kopel} and Ondrej {Závodský} and Jakub {Kapuš} and Libor {Lenža} and Milan {Řezka} and Vojtěch {Adam} and René {Kizek}",
  title="3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase",
  journal="Monatshefte für Chemie",
  year="2016",
  volume="147",
  number="5",
  pages="873--880",
  doi="10.1007/s00706-016-1705-y",
  issn="1434-4475"
}