Age affects not only metabolome but also metal toxicity in Scenedesmus quadricauda cultures

článek v časopise ve Web of Science, Jimp

angličtina

Responses of Scenedesmus quadricauda grown in vitro and differing in age (old culture—13 months, youngculture—1 month) to short-term cadmium (Cd) or nickel (Ni) excess (24 h) were compared. Higher age ofthe culture led to lower amount of chlorophylls, ascorbic acid and glutathione but higher signal of ROS.Surprisingly, sucrose was detected using DART-Orbitrap MS in both old and young culture and subsequentquantification confirmed its higher amount (ca. 3-times) in the old culture. Cd affected viability and ROSamount more negatively than Ni that could arise from excessive Cd uptake which was also higher in alltreatments than in respective Ni counterparts. Surprisingly, nitric oxide was not extensively different inresponse to age or metals. Strong induction of phytochelatin 2 is certainly Cd-specific response while Nialso elevated ascorbate content. Krebs cycle acids were more accumulated in the young culture but theywere rather elevated in the old culture (citric acid under Ni excess). We conclude that organic solid ‘MilieuBristol’ medium we tested is suitable for long-term storage of unicellular green algae (also successfullytested for Coccomyxa sp. and Parachlorella sp.) and the impact of age on metal uptake may be useful forbioremediation purposes.

Aging, Antioxidants, Confocal microscopy, DART-Orbitrap MS, Oxidative stress

KOVÁČIK, J.; KLEJDUS, B.; BABULA, P.; HEDBÁVNÝ, J.

5. 4. 2016

0304-3894

Journal of Hazardous Materials

306

1

Nizozemsko

58

66

9