Detail publikace

An evaluation of different coatings for TiN microelectrode chambers used for neonatal cardiomyocytes

SVOBODA, O. SKOPALÍK, J. BAIAZITOVA, L. GABRIELOVÁ, E. ČMIEL, V. PROVAZNÍK, I. FOHLEROVÁ, Z. HUBÁLEK, J.

Originální název

An evaluation of different coatings for TiN microelectrode chambers used for neonatal cardiomyocytes

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

Neonatal myocytes are a widely used model for modern in-vitro pharmacological screening and tissue engineering of cardiac tissue. The recording of neonatal myocyte electrophysiology in real time has still not been ideally optimised, however. The aim of this study was to evaluate the impact of using different substrates in culture (MEA) chambers on the surviving and functional electrophysiological activity of the myocytes after the first five days after isolation. Neonatal cardiomyocytes were isolated from two day-old neonatal rats and plated on 12×12 ITO microelectrode chambers (Multi-Channel Systems) and cultured for five days. Four coating methods were used. The PDL + fibronectin treatment displayed the highest cardiomyocyte adhesion to the electrodes (20% of total cells in chamber after seeding) and developed contractible cells during the five days of pre-cultivation with a measurable electrical response. Nevertheless, the cells adhered to all surface-treated MEA chambers, creating typical cell morphology, but with different success rates.

Klíčová slova

neonatal cardiomyocyte, microelectrode array, extracellular characterization

Autoři

SVOBODA, O.; SKOPALÍK, J.; BAIAZITOVA, L.; GABRIELOVÁ, E.; ČMIEL, V.; PROVAZNÍK, I.; FOHLEROVÁ, Z.; HUBÁLEK, J.

Vydáno

14. 9. 2016

Nakladatel

Computing in Cardiology

Místo

Vancouver, Canada

ISBN

978-1-5090-0684-7

Kniha

Computing in Cardiology 2016

Edice

43

ISSN

0276-6574

Periodikum

Computers in Cardiology

Ročník

1

Číslo

1

Stát

Spojené státy americké

Strany od

365

Strany do

368

Strany počet

4

URL

https://ieeexplore.ieee.org/document/7868755

BibTex

@inproceedings{BUT128300,
  author="Ondřej {Svoboda} and Josef {Skopalík} and Larisa {Chmelíková} and Eva {Gabrielová} and Vratislav {Čmiel} and Valentine {Provazník} and Zdenka {Fohlerová} and Jaromír {Hubálek}",
  title="An evaluation of different coatings for TiN microelectrode chambers used for neonatal cardiomyocytes",
  booktitle="Computing in Cardiology 2016",
  year="2016",
  series="43",
  journal="Computers in Cardiology",
  volume="1",
  number="1",
  pages="365--368",
  publisher="Computing in Cardiology",
  address="Vancouver, Canada",
  isbn="978-1-5090-0684-7",
  issn="0276-6574",
  url="https://ieeexplore.ieee.org/document/7868755"
}