Detail publikace

Electrochemical Methods for Study of Influence of Selenium Nanoparticles on Antioxidant Status of Rats

HORKÝ, P. RUTTKAY-NEDECKÝ, B. NEJDL, L. RICHTERA, L. CERNEI, N. POHANKA, M. KOPEL, P. SKLÁDANKA, J. HLOUCALOVÁ, P. SLÁMA, P. NEVRKLA, P. MLEJNKOVÁ, V. KLUSOŇOVÁ, I. KIZEK, R. ADAM, V.

Originální název

Electrochemical Methods for Study of Influence of Selenium Nanoparticles on Antioxidant Status of Rats

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The aim of the experiment was to determine the effect of selenium nanoparticles (SeN) and selenium nanoparticles bound with glucose (SeN-GLU) on the antioxidant status of rats. The rats were fed with two defined forms of selenium nanoparticles for ten days. The first experimental group (n=6) was dosed with SeN solution (0.06 mg of Se per kg of body weight / day). The second experimental group (n=6) was dosed with SeN-GLU (0.06 mg of Se and 0.3 mg of glucose per kg of body weight / day). In both experimental groups, the antioxidant activity was decreased in rat plasma and increased in liver, when measured using the both free radicals (FR) and Ferric Reducing Antioxidant Power (FRAP) method. Electrochemical technique (differential pulse voltammetry-DPV) was applied to analyze the content of metallothionein (MT) and heavy metals. The reduced and oxidized forms of glutathione were analyzed by high performance liquid chromatography with electrochemical detection (HPLC-ED). In the analysis of whole blood, a significant increase of reduced glutathione (GSH) by 41% (P < 0.05) was observed in the SeN-GLU group compared with the control group. Higher levels of GSH were also observed in the SeN group by 12%, but without any statistical significance. On the other hand, a smaller increase in oxidized glutathione (GSSG) by 22% (P < 0.05) was observed for the SeN-GLU group and an insignificant decrease by 13% was recorded for the SeN group which implies increased GSH/GSSG ratio and higher antioxidant activity for both SeN-GLU and SeN groups in the whole blood. In the assessing of GSH in erythrocytes, a linear increase was observed for each group.

Klíčová slova

selenium nanoparticles; antioxidant; rat; animal nutrition; selenium; supplementation

Autoři

HORKÝ, P.; RUTTKAY-NEDECKÝ, B.; NEJDL, L.; RICHTERA, L.; CERNEI, N.; POHANKA, M.; KOPEL, P.; SKLÁDANKA, J.; HLOUCALOVÁ, P.; SLÁMA, P.; NEVRKLA, P.; MLEJNKOVÁ, V.; KLUSOŇOVÁ, I.; KIZEK, R.; ADAM, V.

Vydáno

1. 4. 2016

Nakladatel

ESG

ISSN

1452-3981

Periodikum

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Ročník

11

Číslo

4

Stát

Srbská republika

Strany od

2799

Strany do

2824

Strany počet

26

URL

http://electrochemsci.org/papers/vol11/110402799.pdf

Plný text v Digitální knihovně

http://hdl.handle.net/11012/204351

BibTex

@article{BUT128499,
  author="Pavel {Horký} and Branislav {Ruttkay-Nedecký} and Lukáš {Nejdl} and Lukáš {Richtera} and Natalia Vladimirovna {Cernei} and Miroslav {Pohanka} and Pavel {Kopel} and Jiří {Skládanka} and Pavlína {Hloucalová} and Petr {Sláma} and Pavel {Nevrkla} and Veronika {Mlejnková} and Iva {Klusoňová} and René {Kizek} and Vojtěch {Adam}",
  title="Electrochemical Methods for Study of Influence of Selenium Nanoparticles on Antioxidant Status of Rats",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2016",
  volume="11",
  number="4",
  pages="2799--2824",
  issn="1452-3981",
  url="http://electrochemsci.org/papers/vol11/110402799.pdf"
}