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NEUŽIL P. , AHRBERG C. D., MANZ A.
Originální název
Palm-Sized Device for Point-of-Care Ebola Detection
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
We show the utilization of a recently developed cellphone-sized real-time polymerase chain reaction (PCR) device to detect Ebola virus RNA using single-step reverse transcription PCR (RT-PCR). The device was shown to concurrently perform four PCRs, each with a sample volume of 100 nL: one positive control with both Ebola and GAPDH RNA and one negative control. The last two positions were used to measure the GAPDH and the Ebola content of a sample. A comparison of threshold cycles (CT) from the two samples provided relative quantification. The entire process, which consisted of reverse transcription, PCR amplification, and melting curve analysis (MCA), was conducted in less than 37 min. The next step will be integration with a sample preparation unit to form an integrated sample-to-answer system for point-of-care infectious disease diagnostics.
Klíčová slova
REVERSE TRANSCRIPTION-PCR; HEMORRHAGIC-FEVER; VIRUS DISEASE; RAPID DETECTION; CONGO; DIAGNOSIS; OUTBREAK; BLOOD; FIELD; CHIP
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Vydáno
3. 5. 2016
Nakladatel
American Chemical Society
Místo
USA
ISSN
1520-6882
Periodikum
Analytical Chemistry
Ročník
88
Číslo
9
Stát
Spojené státy americké
Strany od
4803
Strany do
4807
Strany počet
5
URL
http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b00278
BibTex
@article{BUT128960, author="Pavel {Neužil} and Christian D. {Ahrberg} and Andreas {Manz}", title="Palm-Sized Device for Point-of-Care Ebola Detection", journal="Analytical Chemistry", year="2016", volume="88", number="9", pages="4803--4807", doi="10.1021/acs.analchem.6b00278", issn="1520-6882", url="http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b00278" }