Detail publikace

On fragmenting, densely mineralised acellular protrusions into articular cartilage and their possible role in osteoarthritis

Boyde, A. Davis, GR. Mills, D. Zikmund, T. Cox, TM. Adams, VL. Niker, A. Wilson, PJ. Dillon, JP. Ranganath, LR. Jeffery, N. Jarvis, JC. Gallagher, JA.

Originální název

On fragmenting, densely mineralised acellular protrusions into articular cartilage and their possible role in osteoarthritis

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

High density mineralised protrusions (HDMP) from the tidemark mineralising front into hyaline articular cartilage (HAC) were first described in Thoroughbred racehorse fetlock joints and later in Icelandic horse hock joints. We now report them in human material. Whole femoral heads removed at operation for joint replacement or from dissection room cadavers were imaged using magnetic resonance imaging (MRI) dual echo steady state at 0.23 mm resolution, then 26-lm resolution high contrast X-ray microtomography, sectioned and embedded in polymethylmethacrylate, blocks cut and polished and re-imaged with 6-lm resolution X-ray microtomography. Tissue mineralisation density was imaged using backscattered electron SEM (BSE SEM) at 20 kV with uncoated samples. HAC histology was studied by BSE SEM after staining block faces with ammonium triiodide solution. HDMP arise via the extrusion of an unknown mineralisable matrix into clefts in HAC, a process of acellular dystrophic calcification. Their formation may be an extension of a crack self-healing mechanism found in bone and articular calcified cartilage. Mineral concentration exceeds that of articular calcified cartilage and is not uniform. It is probable that they have not been reported previously because they are removed by decalcification with standard protocols. Mineral phase morphology frequently shows the agglomeration of many fine particles into larger concretions. HDMP are surrounded by HAC, are brittle, and show fault lines within them. Dense fragments found within damaged HAC could make a significant contribution to joint destruction. At least larger HDMP can be detected with the best MRI imaging ex vivo.

Klíčová slova

dystrophic calcification; hip; hyaline cartilage; microscopy; osteoarthritis; subchondral bone.

Autoři

Boyde, A. ; Davis, GR.; Mills, D.; Zikmund, T.; Cox, TM.; Adams, VL.; Niker, A.; Wilson, PJ.; Dillon, JP.; Ranganath, LR.; Jeffery, N.; Jarvis, JC. ; Gallagher, JA.

Rok RIV

2014

Vydáno

1. 10. 2014

ISSN

0021-8782

Periodikum

JOURNAL OF ANATOMY

Ročník

225

Číslo

4

Stát

Spojené království Velké Británie a Severního Irska

Strany od

436

Strany do

446

Strany počet

11

URL

https://onlinelibrary.wiley.com/doi/abs/10.1111/joa.12226

BibTex

@article{BUT131484,
  author="Boyde, A. and Davis, GR. and Mills, D. and Zikmund, T. and Cox, TM. and Adams, VL. and Niker, A. and Wilson, PJ. and Dillon, JP. and Ranganath, LR. and Jeffery, N. and Jarvis, JC. and Gallagher, JA.",
  title="On fragmenting, densely mineralised acellular protrusions into articular cartilage and their possible role in osteoarthritis",
  journal="JOURNAL OF ANATOMY",
  year="2014",
  volume="225",
  number="4",
  pages="436--446",
  doi="10.1111/joa.12226",
  issn="0021-8782",
  url="https://onlinelibrary.wiley.com/doi/abs/10.1111/joa.12226"
}