The study of biocompatibility of magnetic particles using amplification methods

abstrakt

angličtina

Magnetic particles for biotechnology applications usually consist of a number of components: magnetic cores are covered with a variety of functionalized low-or high- molecular compounds (polymer layer). Iron oxides and some substances used to stabilize magnetic cores can reduce biocompatibility of the proposed particles; therefore perfectly encapsulated cores are very important for their application. The real-time polymerase chain reaction (qPCR) is very sensitive for the presence a number of chemicals. The increasing inhibitors concentration decreases the amplification efficiency and thus the reaction curve slope decreases. The experiments for the estimation magnetic nano- and microparticles inhibitory effect or biocompatibility respectively on the course of real-time PCR were proposed. The set of magnetic non-porous microspheres covered by different content of carboxyl groups, PEGylated monodisperse magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres, newly synthetized types of particles - lanthanide-doped up converting nanoparticles -NaYF4:Yb3+/Er3+ particles and compounds used for magnetic core encapsulation were studied. Linear regression analyses were used for the evaluation of the influence of the particles or compounds on the qPCR course. It was confirmed that the qPCR can be used for the study of compatibility of magnetic particles with DNA amplification. Real-time PCR methods based on shift of Cq values after addition of different amounts of the particles to DNA was found as a suitable method for evaluation of their inhibition effect.

biocompatibility. magnetic nanoparticles, real-time PCR

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; HLÍDKOVÁ, H.; KOSTIV, U.; RITTICH, B.

2. 9. 2015

Brno, Czech Republic

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http://www.ceitec.eu/a5-abstracts-book-3d/f33102