Detail publikace

Real-time polymerase chain reaction as a tool for evaluation of magnetic poly(glycidyl methacrylate)-based microspheres in molecular diagnostics

TRACHTOVÁ, Š. ŠPANOVÁ, A. HORÁK, D. KOZÁKOVÁ, H. RITTICH, B.

Originální název

Real-time polymerase chain reaction as a tool for evaluation of magnetic poly(glycidyl methacrylate)-based microspheres in molecular diagnostics

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

DNA amplification by real-time polymerase chain reaction (RT-PCR) was used for the evaluation of efficiency of polymer coating of magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) and poly(glycidyl methacrylate) (PGMA) microspheres with/without carboxyl groups. The inhibition effect of magnetic microspheres on real-time polymerase chain reaction (RT-PCR) course was evaluated by regression analysis after the addition of different concentrations of tested microspheres to PCR mixtures. Microspheres mostly did not interfere in RT-PCR till the concentration 50 µg/25µl PCR mixture. No relationship between Fe content (and microsphere diameter) and inhibition effect was found. Microspheres containing carboxyl groups extinguished the fluorescence at lower concentrations (10-20 µg/25µl PCR mixture) without inhibition of DNA amplification as PCR products were detected using agarose gel electrophoresis. Negative effect of maghemite on PCR course was partially reduced by coating of magnetic core by silica or polymers. Two inhibition mechanisms of DNA amplification were discussed in this work.

Klíčová slova

magnetic microspheres; inhibitory effect; real-time polymerase chain reaction; regression analysis

Autoři

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; KOZÁKOVÁ, H.; RITTICH, B.

Vydáno

27. 1. 2016

Nakladatel

BENTHAM SCIENCE PUBL LTD

Místo

U ARAB EMIRATES

ISSN

1381-6128

Periodikum

CURRENT PHARMACEUTICAL DESIGN

Ročník

22

Číslo

5

Stát

Stát Spojené arabské emiráty

Strany od

639

Strany do

646

Strany počet

8

URL

http://www.eurekaselect.com/138077/article

BibTex

@article{BUT133353,
  author="Štěpánka {Trachtová} and Alena {Španová} and Daniel {Horák} and Hana {Kozáková} and Bohuslav {Rittich}",
  title="Real-time polymerase chain reaction as a tool for evaluation of magnetic poly(glycidyl methacrylate)-based microspheres in molecular diagnostics",
  journal="CURRENT PHARMACEUTICAL DESIGN",
  year="2016",
  volume="22",
  number="5",
  pages="639--646",
  doi="10.2174/1381612822666151228105006",
  issn="1381-6128",
  url="http://www.eurekaselect.com/138077/article"
}