Detail publikace

Optimization of Enzymatic Hydrolysis of Waste Bread before Fermentation

HUDEČKOVÁ, H. ŠUPINOVÁ, P. BABÁK, L.

Originální název

Optimization of Enzymatic Hydrolysis of Waste Bread before Fermentation

Typ

článek v časopise ve Scopus, Jsc

Jazyk

angličtina

Originální abstrakt

Finding of optimal hydrolysis conditions is important for increasing the yield of saccharides. The higher yield of saccharides is usable for increase of the following fermentation effectivity. In this study optimal conditions (pH and temperature) for amylolytic enzymes were searched. As raw material was used waste bread. Two analytical methods for analysis were used. Efficiency and process of hydrolysis was analysed spectrophotometrically by Somogyi-Nelson method. Final yields of glucose were analysed by HPLC. As raw material was used waste bread from local cafe. Waste bread was pretreated by grinding into small particles. Hydrolysis was performed in 100 mL of 15 % (w/v) waste bread particles in the form of water suspension. Waste bread was hydrolysed by two commercial enzymes. For the liquefaction was used α‑amylase (BAN 240 L). The saccharification was performed by glucoamylase (AMG 300 L). Optimal conditions for α‑amylase (pH 6; 80 °C) were found. The yield of total sugars was 67.08 g∙L-1 (calculated to maltose). As optimal conditions for glucoamylase (pH 4.2; 60 °C) were found. Amount of glucose was 70.28 g∙L1. The time of waste bread liquefaction was 180 minutes. The time of saccharification was 90 minutes. The results were presented at the conference CECE Junior 2014.

Klíčová slova

ethanol, waste bread, enzymatic hydrolysis, amylases, alpha-amylase, glucoamylase, pH, temperature

Autoři

HUDEČKOVÁ, H.; ŠUPINOVÁ, P.; BABÁK, L.

Vydáno

28. 2. 2017

Nakladatel

Mendel University Press

Místo

Brno

ISSN

1211-8516

Periodikum

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis

Ročník

65

Číslo

1

Stát

Česká republika

Strany od

35

Strany do

40

Strany počet

6

URL

https://acta.mendelu.cz/65/1/0035/

Plný text v Digitální knihovně

http://hdl.handle.net/11012/68379

BibTex

@article{BUT133640,
  author="Helena {Hudečková} and Petra {Šupinová} and Libor {Babák}",
  title="Optimization of Enzymatic Hydrolysis of Waste Bread before Fermentation",
  journal="Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis",
  year="2017",
  volume="65",
  number="1",
  pages="35--40",
  doi="10.11118/actaun201765010035",
  issn="1211-8516",
  url="https://acta.mendelu.cz/65/1/0035/"
}