Detail publikace

Antibody-free detection of infectious bacteria using quantum dots-based barcode assay

ČÍHALOVÁ, K. HEGEROVÁ, D. JIMENEZ JIMENEZ, A. MILOSAVLJEVIĆ, V. KUDR, J. SKALIČKOVÁ, S. HYNEK, D. KOPEL, P. VACULOVIČOVÁ, M. ADAM, V.

Originální název

Antibody-free detection of infectious bacteria using quantum dots-based barcode assay

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae are the mostrepresentative bacteria causing infectious diseases. Due to the increased application of antibiotics, thebacterial resistance is growing causing severe complications. Therefore, a sensitive determination of thesepathogens is crucial for effective treatment.The aim of this study was to design an effective method for multiplex detection of Staphylococcus aureus,methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae taking advantage from propertiesof magnetic particles as well as fluorescent nanoparticles (quantum dots). The method was able to detectas low concentrations of bacteria as 102CFU/mL using the bacteria-specific genes (fnbA, mecA and wcaG).

Klíčová slova

Staphylococcus aureus; Methicillin-resistant; Staphylococcus aureus; Klebsiella pneumoniae; Magnetic particles; Barcode; Quantum dots

Autoři

ČÍHALOVÁ, K.; HEGEROVÁ, D.; JIMENEZ JIMENEZ, A.; MILOSAVLJEVIĆ, V.; KUDR, J.; SKALIČKOVÁ, S.; HYNEK, D.; KOPEL, P.; VACULOVIČOVÁ, M.; ADAM, V.

Vydáno

5. 2. 2017

ISSN

0731-7085

Periodikum

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Ročník

134

Číslo

1

Stát

Nizozemsko

Strany od

325

Strany do

332

Strany počet

8

BibTex

@article{BUT133964,
  author="ČÍHALOVÁ, K. and HEGEROVÁ, D. and JIMENEZ JIMENEZ, A. and MILOSAVLJEVIĆ, V. and KUDR, J. and SKALIČKOVÁ, S. and HYNEK, D. and KOPEL, P. and VACULOVIČOVÁ, M. and ADAM, V.",
  title="Antibody-free detection of infectious bacteria using quantum dots-based barcode assay",
  journal="JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS",
  year="2017",
  volume="134",
  number="1",
  pages="325--332",
  doi="10.1016/j.jpba.2016.10.025",
  issn="0731-7085"
}