Detail publikace

Gold nanoparticles-modified nanomaghemite and quantum dots-based hybridization assay for detection of HPV

JIMENEZ JIMENEZ, A. MERLOS RODRIGO, M. MILOSAVLJEVIĆ, V. KŘÍŽKOVÁ, S. KOPEL, P. HEGER, Z. ADAM, V.

Originální název

Gold nanoparticles-modified nanomaghemite and quantum dots-based hybridization assay for detection of HPV

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Head and neck squamous cell carcinomas (HNSCC) are malignant tumors originating from the upperaerodigestive tract. The environmental pollution, high alcohol consumption and smoking are associ-ated with this type of cancer, as well as the human papillomavirus (HPV), which is often involved inthe carcinogenesis and significantly correlates with the cancer prognosis. In recent decades, the num-ber of techniques based on diagnostic of HPV using nanotechnologies including nanoparticles and theirsurface modification has been developed. Herein, we describe specific biosensing system for detectionof HPV. The biosensor employs gold-coated nanoparticles-coated superparamagnetic nanomaghemite(AuMNPs) covered with thiolated oligodeoxyribonucleotides complementary to DNA sequence of E6HPV-16 oncogene. Successful binding of viral ssDNA was recognized by anti-dsDNA antibodies labeledwith HWRGWVC peptide-modified-CdTe quantum dots. The recognition and detection parts were char-acterized and the specificity was tested using HIV, influenza and HBV-specific oligonucleotides. As aproof-of-concept, system was employed for a detection of HPV-16 E6 presence in DNA obtained by eightsubjects with histologically confirmed HNSCC. The obtained results were successfully verified using poly-merase chain reaction (PCR). The developed system offers a cheap alternative to conventional PCR witha comparable sensitivity and versatility.

Klíčová slova

Human papillomavirus; Magnetic isolation; Gold nanoparticle; Nanotechnology; Quantum dots

Autoři

JIMENEZ JIMENEZ, A.; MERLOS RODRIGO, M.; MILOSAVLJEVIĆ, V.; KŘÍŽKOVÁ, S.; KOPEL, P.; HEGER, Z.; ADAM, V.

Vydáno

1. 3. 2017

ISSN

0925-4005

Periodikum

Sensors and Actuators B: Chemical

Ročník

240

Číslo

1

Stát

Švýcarská konfederace

Strany od

503

Strany do

510

Strany počet

8

BibTex

@article{BUT134052,
  author="Ana Maria {Jimenez Jimenez} and Miguel Ángel {Merlos Rodrigo} and Vedran {Milosavljević} and Soňa {Křížková} and Pavel {Kopel} and Zbyněk {Heger} and Vojtěch {Adam}",
  title="Gold nanoparticles-modified nanomaghemite and quantum dots-based hybridization assay for detection of HPV",
  journal="Sensors and Actuators B: Chemical",
  year="2017",
  volume="240",
  number="1",
  pages="503--510",
  doi="10.1016/j.snb.2016.08.091",
  issn="0925-4005"
}