Detail publikace

The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins–Metal Complexes

MERLOS RODRIGO, M. LÓPEZ MOLINA, J. JIMENEZ JIMENEZ, A. PLANELLS DEL POZO, E. ADAM, P. ECKSCHLAGER, T. ZÍTKA, O. RICHTERA, L. ADAM, V.

Originální název

The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins–Metal Complexes

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The translation of metallothioneins (MTs) is one of the defense strategies by which organisms protect themselves from metal-induced toxicity. MTs belong to a family of proteins comprising MT-1, MT-2, MT-3, and MT-4 classes, with multiple isoforms within each class. The main aim of this study was to determine the behavior of MT in dependence on various externally modelled environments, using electrochemistry. In our study, the mass distribution of MTs was characterized using MALDI-TOF. After that, adsorptive transfer stripping technique with differential pulse voltammetry was selected for optimization of electrochemical detection of MTs with regard to accumulation time and pH effects. Our results show that utilization of 0.5 M NaCl, pH 6.4, as the supporting electrolyte provides a highly complicated fingerprint, showing a number of non-resolved voltammograms. Hence, we further resolved the voltammograms exhibiting the broad and overlapping signals using curve fitting. The separated signals were assigned to the electrochemical responses of several MT complexes with zinc(II), cadmium(II), and copper(II), respectively. Our results show that electrochemistry could serve as a great tool for metalloproteomic applications to determine the ratio of metal ion bonds within the target protein structure, however, it provides highly complicated signals, which require further resolution using a proper statistical method, such as curve fitting.

Klíčová slova

electrochemistry; mass spectrometry MALDI-TOF; metallothionein; metallomics; signal resolving

Autoři

MERLOS RODRIGO, M.; LÓPEZ MOLINA, J.; JIMENEZ JIMENEZ, A.; PLANELLS DEL POZO, E.; ADAM, P.; ECKSCHLAGER, T.; ZÍTKA, O.; RICHTERA, L.; ADAM, V.

Vydáno

1. 3. 2017

Nakladatel

MDPI

ISSN

1422-0067

Periodikum

International Journal of Molecular Sciences

Ročník

18

Číslo

3

Stát

Švýcarská konfederace

Strany od

1

Strany do

11

Strany počet

11

URL

http://www.mdpi.com/1422-0067/18/3/610

Plný text v Digitální knihovně

http://hdl.handle.net/11012/69371

BibTex

@article{BUT135984,
  author="MERLOS RODRIGO, M. and LÓPEZ MOLINA, J. and JIMENEZ JIMENEZ, A. and PLANELLS DEL POZO, E. and ADAM, P. and ECKSCHLAGER, T. and ZÍTKA, O. and RICHTERA, L. and ADAM, V.",
  title="The Application of Curve Fitting on the Voltammograms of Various Isoforms of Metallothioneins–Metal Complexes",
  journal="International Journal of Molecular Sciences",
  year="2017",
  volume="18",
  number="3",
  pages="1--11",
  doi="10.3390/ijms18030610",
  issn="1422-0067",
  url="http://www.mdpi.com/1422-0067/18/3/610"
}