Carbon Dots Based FRET for the Detection of DNA Damage

článek v časopise ve Web of Science, Jimp

angličtina

Here, we aimed our attention at the synthesis of carbon dots (C-dots) with the ability to interact with DNA to suggest an approach for the detection of DNA damage. Primarily, C-dots modified with amine moieties were synthesized using the one-step microwave pyrolysis of citric acid in the presence of diethylenetriamine. The Cdots showed strong photoluminescence with a quantum yield of 4%. In addition, the C-dots (2.8 ± 0.8 nm) possessed a good colloidal stability and exhibited a positive surface charge (ζ=36 mV) at a neutral pH. An interaction study of the C-dots and the DNA fragment of λ bacteriophage was performed, and the DNA binding resulted in changes to the photoluminescent and absorption properties of the C-dots. A binding of the C-dots to DNA was also observed as a change to DNA electrophoretic mobility and a decreased ability to intercalate ethidium bromide (EtBr). Moreover, the Förster (or fluorescence) resonance energy transfer (FRET) between the C-dots and EtBr was studied, in which the C-dots serve as an excitation energy donor and the EtBr serves as an acceptor. When DNA was damaged using ultraviolet (UV) radiation (λ=254 nm) and hydroxyl radicals, the intensity of the emitted photoluminescence at 612 nm significantly decreased. The concept was proved on analysis of the genomic DNA from PC-3 cells and DNA isolated from melanoma tissues.

Biosensing; Fluorescence resonance energy transfer; Quantum dots; UV irradiation

KUDR, J.; RICHTERA, L.; XHAXHIU, K.; HYNEK, D.; HEGER, Z.; ZÍTKA, O.; ADAM, V.

15. 6. 2017

0956-5663

BIOSENSORS & BIOELECTRONICS

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Nizozemsko

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