Detail publikace

Application of magnetic polymethacrylate-based microspheres for the isolation of DNA from raw vegetables and processed foods of plant origin

TROJÁNEK, Z. KOVAŘÍK, A. ŠPANOVÁ, A. MAROŠIOVÁ, K. HORÁK, D. RITTICH, B.

Originální název

Application of magnetic polymethacrylate-based microspheres for the isolation of DNA from raw vegetables and processed foods of plant origin

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Plant samples are generally characterised by a complex composition containing various inhibitors of PCR. Here we applied magnetic hydrophilic P(HEMA-co-GMA) microspheres to extract DNA from raw vegetables and highly processed foods of plant origin. Proposed small scale PCR-ready DNA extraction protocol was comparable with a silica-based DNeasy Plant Mini Kit (Qiagen) and classical CTAB extraction methods in quality and amplificability of DNA while it is less costly and time consuming.

Klíčová slova

raw vegetables; processed foods; DNA isolation; magnetic P(HEMA-co-GMA) microspheres; qPCR

Autoři

TROJÁNEK, Z.; KOVAŘÍK, A.; ŠPANOVÁ, A.; MAROŠIOVÁ, K.; HORÁK, D.; RITTICH, B.

Vydáno

5. 1. 2018

Nakladatel

John Wiley & Sons, Inc.

Místo

USA

ISSN

1745-4549

Periodikum

Journal of Food Processing and Preservation

Ročník

42

Číslo

1

Stát

Spojené státy americké

Strany od

1

Strany do

8

Strany počet

8

BibTex

@article{BUT137030,
  author="TROJÁNEK, Z. and KOVAŘÍK, A. and ŠPANOVÁ, A. and MAROŠIOVÁ, K. and HORÁK, D. and RITTICH, B.",
  title="Application of magnetic polymethacrylate-based microspheres for the isolation of DNA from raw vegetables and processed foods of plant origin",
  journal="Journal of Food Processing and Preservation",
  year="2018",
  volume="42",
  number="1",
  pages="1--8",
  doi="10.1111/jfpp.13384",
  issn="1745-4549"
}