Detail publikace

Current trends in electrochemical sensing and biosensing of DNA methylation

KREJČOVÁ, L. RICHTERA, L. HYNEK, D. ADAM, V. LABUDA, J.

Originální název

Current trends in electrochemical sensing and biosensing of DNA methylation

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

DNA methylation plays an important role in physiological and pathological processes. Several genetic diseases and most malignancies tend to be associated with aberrant DNA methylation. Among other analytical methods, electrochemical approaches have been successfully employed for characterisation of DNA methylation patterns that are essential for the diagnosis and treatment of particular diseases. This article discusses current trends in the electrochemical sensing and biosensing of DNA methylation. Particularly, it provides an overview of applied electrode materials, electrode modifications and biorecognition elements applications with an emphasis on strategies that form the core DNA methylation detection approaches. The three main strategies as (i) bisulfite treatment, (ii) cleavage by restriction endonucleases, and (iii) immuno/affinity reaction were described in greater detail. Additionally, the availability of the reviewed platforms for early cancer diagnosis and the approval of methylation inhibitors for anticancer therapy were discussed.

Klíčová slova

Nucleic acids; DNA methylation; Sensor and biosensor; Electrochemistry; Cancer diagnosis

Autoři

KREJČOVÁ, L.; RICHTERA, L.; HYNEK, D.; ADAM, V.; LABUDA, J.

Vydáno

15. 11. 2017

ISSN

0956-5663

Periodikum

BIOSENSORS & BIOELECTRONICS

Ročník

97

Číslo

1

Stát

Nizozemsko

Strany od

384

Strany do

399

Strany počet

16

URL

http://www.sciencedirect.com/science/article/pii/S0956566317300659

BibTex

@article{BUT137481,
  author="Ludmila {Krejčová} and Lukáš {Richtera} and David {Hynek} and Vojtěch {Adam} and Jan {Labuda}",
  title="Current trends in electrochemical sensing and biosensing of DNA methylation",
  journal="BIOSENSORS & BIOELECTRONICS",
  year="2017",
  volume="97",
  number="1",
  pages="384--399",
  doi="10.1016/j.bios.2017.06.004",
  issn="0956-5663",
  url="http://www.sciencedirect.com/science/article/pii/S0956566317300659"
}