Detail publikace

Exceptional release kinetics and cytotoxic selectivity of oxidised MWCNTs double-functionalised with doxorubicin and prostate-homing peptide

MILOSAVLJEVIĆ, V. KREJČOVÁ, L. GURÁŇ, R. BUCHTELOVÁ, H. WAWRZAK, D. RICHTERA, L. HEGER, Z. KOPEL, P. ADAM, V.

Originální název

Exceptional release kinetics and cytotoxic selectivity of oxidised MWCNTs double-functionalised with doxorubicin and prostate-homing peptide

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Multiwall carbon nanotubes (MWCNTs) are among the frequently studied carbon materials, particularly because of their physical and chemical properties and high potential for application in materials chemistry, industry, and medicine. MWCNTs are very promising as transporters of bioactive molecules because of theiurr electrons and large surface area, which can be easily modified, mostly by the application of inorganic acids for the introduction of carboxylic moieties on the surface. In the present study, we designed an oxidised MWCNTs (oMWCNTs) transporter for the targeted delivery of doxorubicin (Dox). The modification of oMWCNTs with prostate-homing peptide (SMSIARL) promotes increased cytotoxicity for prostate cancer cells. Using advanced analytical techniques, we studied the loading efficiency, stability, and release kinetics of Dox from a oMWCNTs-Dox-Pep nanoconstruct. We show that pH strictly drives Dox release, and imitating the pH of intracellular acidic compartments, 60% of Dox is released from oMWCNTs-Dox-Pep, while in plasma conditions, only a 14% release of Dox was found during 24 h. The nanoconstruct displayed no cytotoxicity in non-malignant prostate cells (PNT1A), while in metastatic prostate cancer cells (LNCaP), the cytotoxic effects were close to the cytotoxicity of free Dox. This indicates that peptide modification promotes interactions with malignant cells, resulting in efficient internalisation into the intracellular region. Overall, we show that oMWCNTs are exceptional platforms for simple and stable non-covalent modification with bioactive molecules.

Klíčová slova

Carbon nanomaterial; Prostate-homing peptide; Drug delivery; Nanomedicine

Autoři

MILOSAVLJEVIĆ, V.; KREJČOVÁ, L.; GURÁŇ, R.; BUCHTELOVÁ, H.; WAWRZAK, D.; RICHTERA, L.; HEGER, Z.; KOPEL, P.; ADAM, V.

Vydáno

1. 8. 2017

ISSN

0927-7765

Periodikum

Colloids and Surfaces B: Biointerfaces

Ročník

156

Číslo

1

Stát

Nizozemsko

Strany od

123

Strany do

132

Strany počet

10

BibTex

@article{BUT138242,
  author="Vedran {Milosavljević} and Ludmila {Krejčová} and Roman {Guráň} and Hana {Michálková} and Dorota {Wawrzak} and Lukáš {Richtera} and Zbyněk {Heger} and Pavel {Kopel} and Vojtěch {Adam}",
  title="Exceptional release kinetics and cytotoxic selectivity of oxidised MWCNTs double-functionalised with doxorubicin and prostate-homing peptide",
  journal="Colloids and Surfaces B: Biointerfaces",
  year="2017",
  volume="156",
  number="1",
  pages="123--132",
  doi="10.1016/j.colsurfb.2017.05.008",
  issn="0927-7765"
}