Detail publikace

Anticancerogenic effect of spices due to phenolic and flavonoid compounds – in vitro evaluation on prostate cells

LACKOVÁ, Z. BUCHTELOVÁ, H. BUCHTOVÁ, Ž. KLEJDUS, B. HEGER, Z. BRTNICKÝ, M. KYNICKÝ, J. ZÍTKA, O. ADAM, V.

Originální název

Anticancerogenic effect of spices due to phenolic and flavonoid compounds – in vitro evaluation on prostate cells

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

This study shows the effects of spices, and their phenolic and flavonoid compounds, on prostate cell lines (PNT1A, 22RV1 and PC3). The results of an MTT assay on extracts from eight spices revealed the strongest inhibitory effects were from black pepper and caraway seed extracts. The strongest inhibitory effect on prostatic cells was observed after the application of extracts of spices in concentration of 12.5 mg·mL−1. An LC/MS analysis identified that the most abundant phenolic and flavonoid compounds in black pepper are 3,4-dihydroxybenzaldehyde and naringenin chalcone, while the most abundant phenolic and flavonoid compounds in caraway seeds are neochlorogenic acid and apigenin. Using an MTT assay for the phenolic and flavonoid compounds from spices, we identified the IC50 value of ~1 mmol·L−1 PNT1A. The scratch test demonstrated that the most potent inhibitory effect on PNT1A, 22RV1 and PC3 cells is from the naringenin chalcone contained in black pepper. From the spectrum of compounds assessed, the naringenin chalcone contained in black pepper was identified as the most potent inhibitor of the growth of prostate cells.

Klíčová slova

apigenin; clonogenic assay; 3,4-dihydroxybenzaldehyde; MTT assay; naringenin chalcone; neochlorogenic acid; prostate cancer; scratch test; spices

Autoři

LACKOVÁ, Z.; BUCHTELOVÁ, H.; BUCHTOVÁ, Ž.; KLEJDUS, B.; HEGER, Z.; BRTNICKÝ, M.; KYNICKÝ, J.; ZÍTKA, O.; ADAM, V.

Vydáno

28. 9. 2017

Nakladatel

MDPI

ISSN

1420-3049

Periodikum

MOLECULES

Ročník

22

Číslo

10

Stát

Švýcarská konfederace

Strany od

1

Strany do

13

Strany počet

13

URL

https://www.mdpi.com/1420-3049/22/10/1626

Plný text v Digitální knihovně

http://hdl.handle.net/11012/70123

BibTex

@article{BUT140412,
  author="Zuzana {Lacková} and Hana {Michálková} and Žaneta {Buchtová} and Bořivoj {Klejdus} and Zbyněk {Heger} and Martin {Brtnický} and Jindřich {Kynický} and Ondřej {Zítka} and Vojtěch {Adam}",
  title="Anticancerogenic effect of spices due to phenolic and flavonoid compounds – in vitro evaluation on prostate cells",
  journal="MOLECULES",
  year="2017",
  volume="22",
  number="10",
  pages="1--13",
  doi="10.3390/molecules22101626",
  issn="1420-3049",
  url="https://www.mdpi.com/1420-3049/22/10/1626"
}