Detail publikace

Antimicrobial agent based on selenium nanoparticles and carboxymethyl cellulose for the treatment of bacterial infections

HEGEROVÁ, D. VESELÝ, R. ČÍHALOVÁ, K. KOPEL, P. MILOSAVLJEVIĆ, V. HEGER, Z. HYNEK, D. GURÁŇ, R. VACULOVIČOVÁ, M. SEDLÁČEK, P. ADAM, V.

Originální název

Antimicrobial agent based on selenium nanoparticles and carboxymethyl cellulose for the treatment of bacterial infections

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Our main objective was to analyse and study the effects of the synthesized composite based on selenium nanoparticles and carboxymethyl cellulose (Cekol), hereinafter denoted as SeNPs-Cekol. Firstly, physico-chemical properties of SeNPs-Cekol were characterized in greater detail (size of nanoparticles-from 50 to 150 nm; content of selenium-278 ppm; pH of composite-5.4-5.6; density-990-1010 kg/m(3)), together with assessment of its stability. In addition, the toxicity and mutagenicity on prokaryotic and eukaryotic cells was successfully evaluated. All of the tested bacterial strains were isolated from wound swabs of infectious patients (n = 300) and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). These strains were consequently exposed to SeNPs-Cekol composite. Almost all of the bacterial strains (n = 63) exhibited inhibition zones larger than 5 mm (limit for sensitivity to antibiotics) after the application of the SeNPs-Cekol (300 mu M). Furthermore, in some tested strains (n = 8 for gram positive (G(+)); n = 4 for gram negative (G(-))) even the inhibition zones larger than 12 mm (limit value for very sensitive bacteria to antibiotics) were observed. Overall, the effects of the composite were higher for the G(+) bacteria in comparison with G(-)bacteria, which are generally more resistant to antimicrobial agents, due to their cell wall structure. Further, we found that mutagenicity of the SeNPs-Cekol was found to be negligible. Even, non-target toxicity tests towards eukaryotic cells did not show any significant inhibition of the cells growth compared to the control. Therefore, it can be concluded that SeNPs-Cekol could be considered to have a potential in treatment of bacterial infections.

Klíčová slova

Clinical Isolates; Composite; Synthesis; Antimicrobial; Inhibition

Autoři

HEGEROVÁ, D.; VESELÝ, R.; ČÍHALOVÁ, K.; KOPEL, P.; MILOSAVLJEVIĆ, V.; HEGER, Z.; HYNEK, D.; GURÁŇ, R.; VACULOVIČOVÁ, M.; SEDLÁČEK, P.; ADAM, V.

Vydáno

1. 7. 2017

ISSN

1550-7033

Periodikum

J BIOMED NANOTECHNOL

Ročník

13

Číslo

7

Stát

Spojené státy americké

Strany od

767

Strany do

777

Strany počet

11

BibTex

@article{BUT141267,
  author="Dagmar {Hegerová} and Radek {Veselý} and Kristýna {Číhalová} and Pavel {Kopel} and Vedran {Milosavljević} and Zbyněk {Heger} and David {Hynek} and Roman {Guráň} and Markéta {Vaculovičová} and Pavel {Sedláček} and Vojtěch {Adam}",
  title="Antimicrobial agent based on selenium nanoparticles and carboxymethyl cellulose for the treatment of bacterial infections",
  journal="J BIOMED NANOTECHNOL",
  year="2017",
  volume="13",
  number="7",
  pages="767--777",
  doi="10.1166/jbn.2017.2384",
  issn="1550-7033"
}