Detail publikace

Focus Ion Beam/Scanning Electron Microscopy Characterization of Osteoclastic Resorption of Calcium Phosphate Substrates

DIEZ-ESCUDERO, A. ESPANOL, M. MONTUFAR JIMENEZ, E. DI POMPO, G. CIAPETTI, G. BALDINI, N. GINEBRA, M.

Originální název

Focus Ion Beam/Scanning Electron Microscopy Characterization of Osteoclastic Resorption of Calcium Phosphate Substrates

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

This article presents the application of dual focused ion beam/scanning electron microscopy (FIB-SEM) imaging for preclinical testing of calcium phosphates with osteoclast precursor cells and how this high-resolution imaging technique is able to reveal microstructural changes at a level of detail previously not possible. Calcium phosphate substrates, having similar compositions but different microstructures, were produced using low-and high-temperature processes (biomimetic calcium-deficient hydroxyapatite [CDHA] and stoichiometric sintered hydroxyapatite, respectively). Human osteoclast precursor cells were cultured for 21 days before evaluating their resorptive potential on varying microstructural features. Alternative to classical morphological evaluation of osteoclasts (OC), FIB-SEM was used to observe the subjacent microstructure by transversally sectioning cells and observing both the cells and the substrates. Resorption pits, indicating OC activity, were visible on the smoother surface of high-temperature sintered hydroxyapatite. FIB-SEM analysis revealed signs of acidic degradation on the grain surface under the cells, as well as intergranular dissolution. No resorption pits were evident on the surface of the rough CDHA substrates. However, whereas no degradation was detected by FIB sections in the material underlying some of the cells, early stages of OC-mediated acidic degradation were observed under cells with more spread morphology. Collectively, these results highlight the potential of FIB to evaluate the resorptive activity of OC, even in rough, irregular, or coarse surfaces where degradation pits are otherwise difficult to visualize.

Klíčová slova

focus ion beam; scanning electron microscopy; osteoclast; resorption; calcium phosphate; bone regeneration

Autoři

DIEZ-ESCUDERO, A.; ESPANOL, M.; MONTUFAR JIMENEZ, E.; DI POMPO, G.; CIAPETTI, G.; BALDINI, N.; GINEBRA, M.

Vydáno

1. 2. 2017

ISSN

1937-3392

Periodikum

Tissue engineering. Part C, Methods.

Ročník

23

Číslo

2

Stát

Spojené státy americké

Strany od

118

Strany do

124

Strany počet

7

BibTex

@article{BUT141923,
  author="Anna {Diez-Escudero} and Montserrat {Espanol} and Edgar Benjamin {Montufar Jimenez} and Gemma {Di Pompo} and Gabriela {Ciapetti} and Nicola {Baldini} and Maria-Pau {Ginebra}",
  title="Focus Ion Beam/Scanning Electron Microscopy Characterization of Osteoclastic Resorption of Calcium Phosphate Substrates",
  journal="Tissue engineering. Part C, Methods.",
  year="2017",
  volume="23",
  number="2",
  pages="118--124",
  doi="10.1089/ten.tec.2016.0361",
  issn="1937-3392"
}