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Detail publikace
DOSTÁLOVÁ, S. KONEČNÁ, R. BLAŽKOVÁ, I. VACULOVIČOVÁ, M. KOPEL, P. KŘÍŽKOVÁ, S. VACULOVIČ, T. ADAM, V. KIZEK, R.
Originální název
APOFERRITIN AS A TARGETED DRUG DELIVERY SYSTEM
Typ
článek ve sborníku ve WoS nebo Scopus
Jazyk
angličtina
Originální abstrakt
Conventional cancer treatment often effects normal cells and has many side effects. These can be addressed by the use of nanomedicine, especially its platform nanotransporters, in which cytostatic drug can be encapsulated. These nanotransporters can be dispersed in tumors through relatively large pores in tumor blood vessels but not in normal blood vessels. The nanotransporters can be coupled with antibodies, thus allowing targeted delivery of drugs. For this coupling we chose the method using linker, small peptide that interacts with Fc region of IgG antibodies, presenting the antigen binding site facing out. The aim of this experiment was to create, characterize and test a nanotransporter based on apoferritin nanocage with encapsulated doxorubicin, modified with specific antibody. As a linker, heptapeptide HWRGWVC was used. Cysteine has known affinity towards gold, two methods of apoferritin surface modification were proposed and better results were obtained with apoferritin modified with gold nanoparticles. The presence of gold on apoferritin was proved by separation in polyacrylamide gel electrophoresis (PAGE), followed by inductively coupled plasma mass spectrometric (ICP-MS) measurement. Enzyme-linked immunosorbent assay (ELISA) was used to prove the apoferritin ability to specifically bind to target cells. Apoferritin retained its ability to open and release doxorubicin in low pH. This is very convenient, since there is lower pH in tumors due to hypoxemia.
Klíčová slova
apoferritin; doxorubicin; theranostics; antibodies; targeted delivery
Autoři
DOSTÁLOVÁ, S.; KONEČNÁ, R.; BLAŽKOVÁ, I.; VACULOVIČOVÁ, M.; KOPEL, P.; KŘÍŽKOVÁ, S.; VACULOVIČ, T.; ADAM, V.; KIZEK, R.
Vydáno
21. 11. 2013
ISBN
978-80-7375-908-7
Kniha
MendelNet 2013
Strany od
908
Strany do
912
Strany počet
5
BibTex
@inproceedings{BUT142442, author="Simona {Dostálová} and Romana {Konečná} and Iva {Blažková} and Markéta {Vaculovičová} and Pavel {Kopel} and Soňa {Křížková} and Tomáš {Vaculovič} and Vojtěch {Adam} and René {Kizek}", title="APOFERRITIN AS A TARGETED DRUG DELIVERY SYSTEM", booktitle="MendelNet 2013", year="2013", pages="908--912", isbn="978-80-7375-908-7" }