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POHANKA, M. ADAM, V. KIZEK, R.
Originální název
An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
The enzyme acetylcholinesterase (AChE) is an important part of cholinergic nervous system, where it stops neurotransmission by hydrolysis of the neurotransmitter acetylcholine. It is sensitive to inhibition by organophosphate and carbamate insecticides, some Alzheimer disease drugs, secondary metabolites such as aflatoxins and nerve agents used in chemical warfare. When immobilized on a sensor (physico-chemical transducer), it can be used for assay of these inhibitors. In the experiments described herein, an AChE- based electrochemical biosensor using screen printed electrode systems was prepared. The biosensor was used for assay of nerve agents such as sarin, soman, tabun and VX. The limits of detection achieved in a measuring protocol lasting ten minutes were 7.41 x 10(-12) mol/L for sarin, 6.31 x 10(-12) mol/L for soman, 6.17 x 10(-11) mol/L for tabun, and 2.19 x 10(-11) mol/L for VX, respectively. The assay was reliable, with minor interferences caused by the organic solvents ethanol, methanol, isopropanol and acetonitrile. Isopropanol was chosen as suitable medium for processing lipophilic samples.
Klíčová slova
biosensor; acetylcholinesterase; sarin; tabun; soman; VX; inhibitor; screen printed electrode; voltammetry; amperometry
Autoři
POHANKA, M.; ADAM, V.; KIZEK, R.
Vydáno
1. 9. 2013
Nakladatel
MDPI
ISSN
1424-8220
Periodikum
SENSORS
Ročník
13
Číslo
9
Stát
Švýcarská konfederace
Strany od
11498
Strany do
11506
Strany počet
7
URL
https://www.mdpi.com/1424-8220/13/9/11498
Plný text v Digitální knihovně
http://hdl.handle.net/11012/137241
BibTex
@article{BUT143113, author="Miroslav {Pohanka} and Vojtěch {Adam} and René {Kizek}", title="An Acetylcholinesterase-Based Chronoamperometric Biosensor for Fast and Reliable Assay of Nerve Agents", journal="SENSORS", year="2013", volume="13", number="9", pages="11498--11506", doi="10.3390/s130911498", issn="1424-8220", url="https://www.mdpi.com/1424-8220/13/9/11498" }