Detail publikace

Tularemia Progression Accompanied with Oxidative Stress and Antioxidant Alteration in Spleen and Liver of BALB/c Mice

POHANKA, M. PAVLIS, O. RUTTKAY-NEDECKÝ, B. SOCHOR, J. SOBOTKA, J. PIKULA, J. ADAM, V. KIZEK, R.

Originální název

Tularemia Progression Accompanied with Oxidative Stress and Antioxidant Alteration in Spleen and Liver of BALB/c Mice

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Francisella tularensis is the causative agent of tularemia. It is an intracellular pathogen with the ability to survive within phagosomes and induce pyroptotic cell death. In this study, we attempted to prove whether oxidative imbalance plays a significant role in tularemia pathogenesis. In our experimental model, we subcutaneously infected female BALB/c mice (dose 10(5) CFU of F. tularensis LVS). Liver, spleen, and blood were collected from mice at regular intervals from days 1-15 after infection. The bacterial burden was assessed by a cultivation test. The burden was unchanging from the 2nd to 6th day after infection. The bacterial burden corresponded to the plasmatic level of IFN-gamma, IL-6, and liver malondialdehyde. After the phase of acute bacteraemia and the innate immunity reaction, the levels of reduced glutathione and total low molecular weight antioxidants decreased significantly and the activity of caspase-3 increased in the liver. The level of reduced glutathione decreased to 25% of the original level, and the total level of low molecular weight antioxidants was less than 50% of the initial amount. The demonstrated effects of tularemia-induced pathology had a more extensive impact on the liver than on the spleen.

Klíčová slova

Francisella tularensis; tularaemia; oxidative stress; inflammation; antioxidant; glutathione

Autoři

POHANKA, M.; PAVLIS, O.; RUTTKAY-NEDECKÝ, B.; SOCHOR, J.; SOBOTKA, J.; PIKULA, J.; ADAM, V.; KIZEK, R.

Vydáno

1. 6. 2012

ISSN

1225-8873

Periodikum

JOURNAL OF MICROBIOLOGY

Ročník

50

Číslo

3

Stát

Korejská republika

Strany od

401

Strany do

408

Strany počet

8

BibTex

@article{BUT145191,
  author="POHANKA, M. and PAVLIS, O. and RUTTKAY-NEDECKÝ, B. and SOCHOR, J. and SOBOTKA, J. and PIKULA, J. and ADAM, V. and KIZEK, R.",
  title="Tularemia Progression Accompanied with Oxidative Stress and Antioxidant Alteration in Spleen and Liver of BALB/c Mice",
  journal="JOURNAL OF MICROBIOLOGY",
  year="2012",
  volume="50",
  number="3",
  pages="401--408",
  doi="10.1007/s12275-012-1621-8",
  issn="1225-8873"
}