Detail publikace

The Anticancer Drug Ellipticine Induces Cytochromes P450 1A1, 1A2 and 3A, Cytochrome b(5) and NADPH:Cytochrome P450 Oxidoreductase in Rat Liver, Kidney and Lung

VRÁNOVÁ, I. MOSEROVÁ, M. HODEK, P. KIZEK, R. FREI, E. STIBOROVÁ, M.

Originální název

The Anticancer Drug Ellipticine Induces Cytochromes P450 1A1, 1A2 and 3A, Cytochrome b(5) and NADPH:Cytochrome P450 Oxidoreductase in Rat Liver, Kidney and Lung

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The antineoplastic alkaloid ellipticine is a prodrug, the pharmacological efficiency of which is dependent on its cytochrome P450 (CYP)- and/or peroxidase-mediated activation in target tissues. Using the Western blotting, we found that this compound increases protein expression of cytochrome b(5), CYP1A1, 1A2, 3A and NADPH: CYP oxidoreductase (POR) in livers, lungs and kidneys of rats treated (i.p.) with ellipticine. The ellipticine-mediated induction of these enzymes resulted in an increase in their enzymatic activities and ellipticine oxidation to 7-hydroxy-, 9-hydroxy-, 12-hydroxy-and 13-hydroxyellipticine, the metabolites that are both detoxication products (7-hydroxy-, 9-hydroxyellipticine) and metabolites responsible for generation ellipticine-derived DNA adducts (12-hydroxy- and 13-hydroxyellipticine). The results demonstrate that by inducing CYP1A1/2, 3A, POR and cytochrome b(5), ellipticine increases its own metabolism in rats, thereby modulating its own pharmacological and/or genotoxic potential.

Klíčová slova

Ellipticine; Cytochromes P450; Protein Expression; Western Blotting; Metabolism

Autoři

VRÁNOVÁ, I.; MOSEROVÁ, M.; HODEK, P.; KIZEK, R.; FREI, E.; STIBOROVÁ, M.

Vydáno

1. 2. 2013

ISSN

1452-3981

Periodikum

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Ročník

8

Číslo

2

Stát

Srbská republika

Strany od

1586

Strany do

1597

Strany počet

12

BibTex

@article{BUT145264,
  author="VRÁNOVÁ, I. and MOSEROVÁ, M. and HODEK, P. and KIZEK, R. and FREI, E. and STIBOROVÁ, M.",
  title="The Anticancer Drug Ellipticine Induces Cytochromes P450 1A1, 1A2 and 3A, Cytochrome b(5) and NADPH:Cytochrome P450 Oxidoreductase in Rat Liver, Kidney and Lung",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2013",
  volume="8",
  number="2",
  pages="1586--1597",
  issn="1452-3981"
}