ELECTROCHEMICAL DETECTION OF MIR-124 ISOLATED BY MAGNETIC PARTICLES

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angličtina

MicroRNAs (miRNAs) are a novel class of single-stranded RNAs, which have an important role in the regulation of gene expression at the posttranscriptional level . These relatively short (~23 nucleotides long) RNA molecules are non-coding and play key role in many cellular processes (proliferation, differentiation, apoptosis, etc.) MiRNAs may have a different expression pattern in a patient with a tumour disease in comparison to healthy subjects [1]. The relations between many miRNAs and different types of cancer have been found [2]. One of them is the miR-124, which is highly expressed in neuronal cells and recently its downregulation in several types of human cancer as breast or prostate cancer was reported [3]. Usually is the detection of miRNAs coupled with the usage of a number of bioanalytical methods as are northern blotting, real-time reverse transcription polymerase chain reaction (RT-qPCR), in situ hybridization (ISH) and micro-RNA arrays [4]. Opposite these, the electrochemical techniques could be used as suitable analytical tool for nucleic acid determination [5, 6]. Especially utilization of electrochemical labels for the target sequence detection is very widespread [7]. Coupling of electrochemical detection of nucleic acids with the appropriate isolation step using nano- and microstructured materials (magnetic particles, quantum dots, etc.) is very promising area research.

MicroRNAs; magnetic particles (MPs); electrochemical detection

VLACHOVÁ, J.; HYNEK, D.; KOUDELKOVÁ, Z.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

1. 1. 2015

978-80-904959-3-7

CECE 2015

217

220

4