Detail publikace

Simultaneous production of lipids and pigments by red yeasts grown on animal fat using the concept of biorefinery

MÁROVÁ, I. SZOTKOWSKI, M. RAPTA, M. BYRTUSOVÁ, D. VANĚK, M. HÁRONIKOVÁ, A. MIKHEICHYK, N.

Originální název

Simultaneous production of lipids and pigments by red yeasts grown on animal fat using the concept of biorefinery

Typ

článek ve sborníku mimo WoS a Scopus

Jazyk

angličtina

Originální abstrakt

Characterization of metabolic activity of yeasts in biotechnological applications is an important issue both for routine analysis and trouble-shooting incidences. In this work 12 different strains of red yeasts (Rhodotorula, Sporobolomyces, Cystofilobasidium) were used for screening of growth and metabolic activity. Simultaneous production of lipids and other lipid metabolites was followed. As a carbon substrate waste animal fat and its hydrolysis products were used according to the biorefinery concept. Yeasts were cultivated in medium with particular C/N ratio. Whey was used as alternative nitrogen source. High-throughput micro-cultivation, FTIR spectroscopy and single cell analysis of red yeasts was used for monitoring of growth characteristics and total metabolic activity of yeasts. Simultaneously, chromatographic techniques were used for quantification of production of lipids and fatty acids (GC/FID), sterols, carotenoids and CoQ (LC/MS). Glycerol, lipase and total lipids were measured by enzyme methods. Cells were monitored by advanced fluorescence techniques on single-cell level.

Klíčová slova

biorefinery, red yeasts, animal fat

Autoři

MÁROVÁ, I.; SZOTKOWSKI, M.; RAPTA, M.; BYRTUSOVÁ, D.; VANĚK, M.; HÁRONIKOVÁ, A.; MIKHEICHYK, N.

Vydáno

18. 10. 2017

Nakladatel

Formatex Research Centre

Místo

Madrid, Spain

Strany od

199

Strany do

202

Strany počet

345

URL

http://biomicroworld2017.org/

BibTex

@inproceedings{BUT145815,
  author="Ivana {Márová} and Martin {Szotkowski} and Marek {Rapta} and Dana {Byrtusová} and Martin {Vaněk} and Andrea {Němcová} and Nadzeya {Mikheichyk}",
  title="Simultaneous production of lipids and pigments by red yeasts grown on animal fat using the concept of biorefinery",
  year="2017",
  pages="199--202",
  publisher="Formatex Research Centre",
  address="Madrid, Spain",
  url="http://biomicroworld2017.org/"
}