Přístupnostní navigace
E-přihláška
Vyhledávání Vyhledat Zavřít
Detail publikace
SEDLAČÍK, T. ACAR, O. STUDENOVSKÁ, H. KOTELNIKOV, I. KUČKA, J. KONEČNÁ, Z. ZIKMUND, T. KAISER, J. KOSE, G. RYPÁČEK, F.
Originální název
Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(a-amino acids)
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(a-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N5-(2-hydroxyethyl)-L-glutamine-stat-N5-(2-methacryloyl-oxyethyl)- L-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers. PrMAAm provided alkyne groups for modifying the gels with cellsupporting moieties (RGDS peptides) by the azide–alkyne ‘‘click’’-reaction. Two types of gels with different compressive moduli were prepared. Each type was modified with two different concentrations of RGDS peptide. X-ray computed nanotomography (nanoCT) was used to visualize and analyze the 3D-structure of the cryogels. It was shown that modifying the PHEG-MA cryogels within the range of RGDS concentrations examined here had a positive effect on the proliferation of hDPSCs. Immunofluorescence staining for collagen type 2 and aggrecan proved that there was differentiation of hDPSCs into chondrocytes.
Klíčová slova
cryogel, polyamino acid
Autoři
SEDLAČÍK, T.; ACAR, O.; STUDENOVSKÁ, H.; KOTELNIKOV, I.; KUČKA, J.; KONEČNÁ, Z.; ZIKMUND, T.; KAISER, J.; KOSE, G.; RYPÁČEK, F.
Vydáno
30. 10. 2017
ISSN
1744-683X
Periodikum
Soft Matter
Číslo
14
Stát
Spojené království Velké Británie a Severního Irska
Strany od
228
Strany do
238
Strany počet
11
BibTex
@article{BUT146251, author="Tomáš {Sedlačík} and O.K. {Acar} and Hana {Studenovská} and Ilyia {Kotelnikov} and Jan {Kučka} and Zuzana {Konečná} and Tomáš {Zikmund} and Jozef {Kaiser} and G.T. {Kose} and František {Rypáček}", title="Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(a-amino acids)", journal="Soft Matter", year="2017", number="14", pages="228--238", doi="10.1039/c7sm02074k", issn="1744-683X" }