Detail publikace

Antibacterial biocompatible PCL nanofibers modified by COOH-anhydride plasma polymers and gentamicin immobilization

PERMYAKOVA, E. POLČÁK, J. SLUKIN, P. IGNATOV, S. GLOUSHANKOVA, N. ZAJÍČKOVÁ, L. SHTANSKY, D. MANAKHOV, A.

Originální název

Antibacterial biocompatible PCL nanofibers modified by COOH-anhydride plasma polymers and gentamicin immobilization

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Herein COOH/anhydride functionalized biodegradable nanofibers were prepared using atmospheric pressure plasma copolymerization of maleic anhydride (MA) and C2H2. The XPS and ATR-FTIR analyses revealed a high concentration of carboxyl and anhydride groups that were used for grafting of gentamycin (GM) onto the surface of PCL nanofibers. It was shown that GM can be immobilized either with or without dicyclohexyl carbodiimde (DCC). The immobilization without DCC led to ionic bonding (PCL-MA-GMi), whereas the immobilization using DCC activation resulted in covalent bonding (PCL-MA-GMc). The investigation of IAR2 epithelial cell adhesion and proliferation revealed that the GM-loaded nanofibers were biocompatible. The nanofibers without immobilized GM did not show any noticeable antibacterial activity against E. coli bacteria with different resistance to gentamicin. In contrast, the size of inhibition zone around the PCL-MA-GMi and PCL-MA-GMc samples reached 27 mm, hereby indicating a strong antibacterial effect against all types of E. coli bacteria. The GM-loaded nanofibers also demonstrated a pronounced antibacterial effect after immersion in phosphate buffered saline at 37 °C for 24 h. Thus the results demonstrated that the proposed strategy for the preparation of antibacterial biocompatible nanofibers with relatively long-term antibacterial protection has a great potential for future application for wound healing.

Klíčová slova

Nanofibers Biomaterials Cell adhesion Antibacterial effect Gentamicin Plasma polymer

Autoři

PERMYAKOVA, E.; POLČÁK, J.; SLUKIN, P.; IGNATOV, S.; GLOUSHANKOVA, N.; ZAJÍČKOVÁ, L.; SHTANSKY, D.; MANAKHOV, A.

Vydáno

5. 9. 2018

ISSN

0261-3069

Periodikum

Materials and design

Ročník

153

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

60

Strany do

70

Strany počet

11

URL

http://www.sciencedirect.com/science/article/pii/S0264127518303794

BibTex

@article{BUT149866,
  author="Elizaveta {Permyakova} and Josef {Polčák} and Pavel V. {Slukin} and Sergei G. {Ignatov} and Natalia A. {Gloushankova} and Lenka {Zajíčková} and Dmitry V. {Shtansky} and Anton {Manakhov}",
  title="Antibacterial biocompatible PCL nanofibers modified by COOH-anhydride plasma polymers and gentamicin immobilization",
  journal="Materials and design",
  year="2018",
  volume="153",
  number="1",
  pages="60--70",
  doi="10.1016/j.matdes.2018.05.002",
  issn="0261-3069",
  url="http://www.sciencedirect.com/science/article/pii/S0264127518303794"
}