Detail publikace

Real-Time Visualization of Cell Membrane Damage Using Gadolinium Schiff Base Complex-Doped Quantum Dots

MOULICK, A. HEGER, Z. MILOSAVLJEVIĆ, V. RICHTERA, L. BARROSO-FLORES, J. MERLOS RODRIGO, M. BUCHTELOVÁ, H. PODGAJNY, R. HYNEK, D. KOPEL, P. ADAM, V.

Originální název

Real-Time Visualization of Cell Membrane Damage Using Gadolinium Schiff Base Complex-Doped Quantum Dots

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Despite the importance of cell membranes for maintenance of integrity of cellular structures, there is still a lack of methods that allow simple real-time visualization of their damage. Herein, we describe gadolinium Schiff base doped quantum dots (GdQDs)-based probes for a fast facile spatial labeling of membrane injuries. We found that GdQDs preferentially interact through electron-rich and hydrophobic residues with a specific sequence motif of NHE-RF2 scaffold protein, exposed upon membrane damage. Such interaction results in a fast formation of intensively fluorescent droplets with a higher resolution and in a much shorter time compared to immunofluorescence using organic dye. GdQDs have high stability, brightness, and considerable cytocompatibility, which enable their use in long-term experiments in living cultures. To the best of our knowledge, this is the first report, demonstrating a method allowing real-time monitoring of membrane damage and recovery without any special requirements for instrumentation. Because of intensive brightness and simple signal pattern, GdQDs allow easy examination of interactions between cellular membranes and cell-penetrating peptides or cytostatic drugs. We anticipate that the simple and flexible method will also facilitate the studies dealing with host-pathogen interactions.

Klíčová slova

cell-penetrating peptides; core-shell; quantum dots; fluorescence labeling; nanotechnologies

Autoři

MOULICK, A.; HEGER, Z.; MILOSAVLJEVIĆ, V.; RICHTERA, L.; BARROSO-FLORES, J.; MERLOS RODRIGO, M.; BUCHTELOVÁ, H.; PODGAJNY, R.; HYNEK, D.; KOPEL, P.; ADAM, V.

Vydáno

24. 10. 2018

ISSN

1944-8244

Periodikum

ACS APPL MATER INTER

Ročník

10

Číslo

42

Stát

Spojené státy americké

Strany od

35859

Strany do

35868

Strany počet

10

BibTex

@article{BUT151220,
  author="Amitava {Moulick} and Zbyněk {Heger} and Vedran {Milosavljević} and Lukáš {Richtera} and Joaquin {Barroso-Flores} and Miguel Ángel {Merlos Rodrigo} and Hana {Michálková} and Robert {Podgajny} and David {Hynek} and Pavel {Kopel} and Vojtěch {Adam}",
  title="Real-Time Visualization of Cell Membrane Damage Using Gadolinium Schiff Base Complex-Doped Quantum Dots",
  journal="ACS APPL MATER INTER",
  year="2018",
  volume="10",
  number="42",
  pages="35859--35868",
  doi="10.1021/acsami.8b15868",
  issn="1944-8244"
}