Detail publikace

Mass spectrometric imaging of cysteine rich proteins in human skin

POMPEIANO VANÍČKOVÁ, L. GURÁŇ, R. KOLLAR, S. EMRI, G. KŘÍŽKOVÁ, S. DO, T. HEGER, Z. ZÍTKA, O. ADAM, V.

Originální název

Mass spectrometric imaging of cysteine rich proteins in human skin

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Looking insight pathological processes, metallothioneins (MTs) are considered to be potential biomarkers for monitoring of a development of various types of diseases, such as cancer. The early identification of the MTs in biological tissues could be important tool for the estimation of appropriate clinical therapy. Therefore, here we investigated the application of matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) together with immunohistochemical analyses (IHC) using MT-1/2 antibody for MT detection in formalin-fixed paraffin-embedded (FFPE) biopsy specimens of human skin. Principal component analyses revealed differences in the peptide/protein profiles separating healthy skin from the carcinoma specimens. Statistically significant ion peaks at m/z 6038, 6300, 6676, and 7026 were more frequently detected in squamous cell carcinoma (SCC), basal cell carcinoma (BCC) and melanoma. Using IHC, we found that MT-1/2 was significantly higher in SCC and melanoma compared to healthy skin. Surprisingly, significantly low levels of MT-1/2 were found in BCC. On one side, the results indicate important role of MTs in melanoma occurrence and progression, as on the second side, there are hidden processes associated with MTs based on differences of the occurrence of the MS peaks, which could be associated with cycling of MTs isoforms.

Klíčová slova

Metallothionein; Squamous cell carcinoma; Melanoma; MALDI MSI; Immunohistochemistry

Autoři

POMPEIANO VANÍČKOVÁ, L.; GURÁŇ, R.; KOLLAR, S.; EMRI, G.; KŘÍŽKOVÁ, S.; DO, T.; HEGER, Z.; ZÍTKA, O.; ADAM, V.

Vydáno

15. 3. 2019

Nakladatel

Elsevier

ISSN

0141-8130

Periodikum

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Ročník

125

Číslo

1

Stát

Nizozemsko

Strany od

270

Strany do

277

Strany počet

8

URL

https://www.sciencedirect.com/science/article/pii/S0141813018356241

Plný text v Digitální knihovně

http://hdl.handle.net/11012/194755

BibTex

@article{BUT156230,
  author="Lucie {Pompeiano Vaníčková} and Roman {Guráň} and Sandor {Kollar} and Gabriela {Emri} and Soňa {Křížková} and Tomáš {Do} and Zbyněk {Heger} and Ondřej {Zítka} and Vojtěch {Adam}",
  title="Mass spectrometric imaging of cysteine rich proteins in human skin",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2019",
  volume="125",
  number="1",
  pages="270--277",
  doi="10.1016/j.ijbiomac.2018.11.272",
  issn="0141-8130",
  url="https://www.sciencedirect.com/science/article/pii/S0141813018356241"
}