Detail publikace

Investigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cells

STRMISKA, V. BUCHTELOVÁ, H. MICHÁLEK, P. KŘÍŽKOVÁ, S. ADAM, V. HEGER, Z.

Originální název

Investigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cells

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

It has been shown that sarcosine supplementation stimulates proliferation and invassivenes of prostate cells. Nevertheless, the exact molecular mechanism responsible for this phenomenon is not known. In the present study we demonstrate that sarcosine increases expression of calmodulin (CaM), an important intracellular signaling molecule. Through this, sarcosine activates calmodulin-dependent protein kinases signaling. Pathway of activation CaM-dependent protein kinases can activate regulation of mitosis, proliferation, cell death, gene transcription and phosphorylation/ dephosphorlation of proteins. This is done through CaM binding of four Ca2+ ions. Interestingly, in this study, we identified decrese in free Ca2+ correlating with sarcosine-induced up-regulation of CaM. The influence of CaM to cell cycle changes was further verified using post transcritrional gene silencing using CaM-siRNA complex. Co-treatment of prostate cells with CaM-siRNA and sarcosine showed decrease in CaM-dependent kinases and cell invasiveness compared to sarcosine treatment only.

Klíčová slova

sarcosine; calmodulin; prostate; prostate cancer; human cells

Autoři

STRMISKA, V.; BUCHTELOVÁ, H.; MICHÁLEK, P.; KŘÍŽKOVÁ, S.; ADAM, V.; HEGER, Z.

Vydáno

2. 1. 2019

Nakladatel

Mendel University in Brno

ISBN

978-80-7509-597-8

Kniha

MendelNet 2018

Strany od

532

Strany do

536

Strany počet

5

URL

https://mendelnet.cz/artkey/mnt-201801-0113_Investigating-the-interplay-between-sarcosine-and-Ca-sup-2-sup-dependent-signaling-in-prostate-cells.php?back=/magno/mnt/2018/mn1.php?secid=4

Plný text v Digitální knihovně

http://hdl.handle.net/11012/178388

BibTex

@inproceedings{BUT156246,
  author="Vladislav {Strmiska} and Hana {Michálková} and Petr {Michálek} and Soňa {Křížková} and Vojtěch {Adam} and Zbyněk {Heger}",
  title="Investigating the interplay between sarcosine and Ca2+-dependent signaling in prostate cells",
  booktitle="MendelNet 2018",
  year="2019",
  pages="532--536",
  publisher="Mendel University in Brno",
  isbn="978-80-7509-597-8",
  url="https://mendelnet.cz/artkey/mnt-201801-0113_Investigating-the-interplay-between-sarcosine-and-Ca-sup-2-sup-dependent-signaling-in-prostate-cells.php?back=/magno/mnt/2018/mn1.php?secid=4"
}