Detail publikace

Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing

VANĚČKOVÁ, T. POMPEIANO VANÍČKOVÁ, L. TVRDOŇOVÁ, M. POMORSKI, A. KRĘŻEL, A. VACULOVIČ, T. KANICKÝ, V. VACULOVIČOVÁ, M. ADAM, V.

Originální název

Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

We report a facile method for detection of metallothionein (MT), a promising clinically relevant biomarker, in spiked plasma samples. This method, for the first time, integrates molecularly imprinted polymers as purification/pretreatment step with matrix assisted laser desorption/ionization time-of-flight mass spectrometric detection and with laser ablation inductively coupled plasma mass spectrometry for analysis of MTs. The prepared MT-imprinted polydopamine layer showed high binding capacity and specific recognition properties toward the template. Optimal monomer (dopamine) concentration was found to be 16 mM of dopamine. This experimental setup allows to measure mu M concentrations of MT that are present in blood as this can be used for clinical studies recognizing MT as marker of various diseases including tumour one. Presented approach not only provides fast sample throughput but also avoids the limitations of methods based on use of antibodies (e.g. high price, cross reactivity, limited availability in some cases, etc.).

Klíčová slova

Metallothionein; Molecularly imprinted polymers; Polydopamine; MALDI-TOP-MS; LA-ICP-MS

Autoři

VANĚČKOVÁ, T.; POMPEIANO VANÍČKOVÁ, L.; TVRDOŇOVÁ, M.; POMORSKI, A.; KRĘŻEL, A.; VACULOVIČ, T.; KANICKÝ, V.; VACULOVIČOVÁ, M.; ADAM, V.

Vydáno

1. 6. 2019

Nakladatel

Elsevier

ISSN

0039-9140

Periodikum

Talanta

Ročník

198

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

224

Strany do

229

Strany počet

6

URL

https://www.sciencedirect.com/science/article/pii/S0039914019301109

Plný text v Digitální knihovně

http://hdl.handle.net/11012/195645

BibTex

@article{BUT156653,
  author="Tereza {Vaněčková} and Lucie {Pompeiano Vaníčková} and Michaela {Kuchynka} and Adam {Pomorski} and Artur {Krężel} and Tomáš {Vaculovič} and Viktor {Kanický} and Markéta {Vaculovičová} and Vojtěch {Adam}",
  title="Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing",
  journal="Talanta",
  year="2019",
  volume="198",
  number="1",
  pages="224--229",
  doi="10.1016/j.talanta.2019.01.089",
  issn="0039-9140",
  url="https://www.sciencedirect.com/science/article/pii/S0039914019301109"
}