Detail publikace

Black Phosphorus Cytotoxicity Assessments Pitfalls: Advantages and Disadvantages of Metabolic and Morphological Assays

FOJTŮ, M. BALVAN, J. RAUDENSKÁ, M. VIČAR, T. BOUŠA, D. SOFER, Z. MASAŘÍK, M. PUMERA, M.

Originální název

Black Phosphorus Cytotoxicity Assessments Pitfalls: Advantages and Disadvantages of Metabolic and Morphological Assays

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Black phosphorus (BP) belongs to a group of 2D nanomaterials and nowadays attracts constantly increasing attention. Parallel to the growing utilization of BP nanomaterial increase also the requirements for the thorough comprehension of its potential impact on human and animal health. The aim of this study was to compare and discuss five assays commonly used for the cytotoxicity assessments of nanomaterials with a special focus on BP nanoparticles. A comprehensive survey of factors and pitfalls is provided that should be accounted for when assessing their toxicity and pointed to their inconsistency. BP might introduce various levels of interference during toxicity assessments depending on its concentration applied. More importantly, the BP toxicity evaluation was found to be influenced by the nature of assay chosen. These are based on different principles and do not have to assess all the cellular events equally. A commercial assay based on the measurement of protease activity was identified to be the most suitable for the BP toxicity assessment. Further, the benefit of time-lapse quantitative phase imaging for nanomaterial toxicity evaluation was highlighted. Unlike the conventional assessments it provides real-time analysis of the processes accompanying BP administration and enables to understand them deeper and in the context.

Klíčová slova

2D nanomaterials; black phosphorus; cytotoxicity; holographic microscopy; interference; nanoparticles; viability

Autoři

FOJTŮ, M.; BALVAN, J.; RAUDENSKÁ, M.; VIČAR, T.; BOUŠA, D.; SOFER, Z.; MASAŘÍK, M.; PUMERA, M.

Vydáno

2. 1. 2019

ISSN

0947-6539

Periodikum

CHEMISTRY-A EUROPEAN JOURNAL

Ročník

25

Číslo

1

Stát

Spolková republika Německo

Strany od

349

Strany do

360

Strany počet

13

URL

https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201804434

BibTex

@article{BUT158443,
  author="Michaela {Fojtů} and Jan {Balvan} and Martina {Raudenská} and Tomáš {Vičar} and Daniel {Bouša 	} and Zdeněk {Sofer} and Michal {Masařík} and Martin {Pumera}",
  title="Black Phosphorus Cytotoxicity Assessments Pitfalls: Advantages and Disadvantages of Metabolic and Morphological Assays",
  journal="CHEMISTRY-A EUROPEAN JOURNAL",
  year="2019",
  volume="25",
  number="1",
  pages="349--360",
  doi="10.1002/chem.201804434",
  issn="0947-6539",
  url="https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201804434"
}