Přístupnostní navigace
E-přihláška
Vyhledávání Vyhledat Zavřít
Detail publikace
SZOTKOWSKI, M. BYRTUSOVÁ, D. NĚMCOVÁ, A. VYSOKÁ, M. RAPTA, M. SHAPAVAL, V. MÁROVÁ, I.
Originální název
Study of Metabolic Adaptation of Red Yeasts to Waste Animal Fat Substrate
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Carotenogenic yeasts are non-conventional oleaginous microorganisms capable to utilize various waste substrates. In this work 4 red yeast strains (Rhodotorula, Cystofilobasidium and Sporobolomyces sp.) were cultivated in media containing crude, emulsified and enzymatically hydrolysed animal waste fat, compared with glucose and glycerol as single C-sources. Cell morphology (cryo-SEM, TEM), production of biomass, lipase, biosurfactants, lipids (GC/FID) carotenoids, ubiquinone, ergosterol (HPLC/PDA) in yeast cells was studied depending on medium composition, C-source and C/N ratio. All studied strains are able to utilize solid and processed fat. Biomass production at C/N=13 was higher on emulsified/hydrolysed fat than on glucose/glycerol. Production of lipids and lipidic metabolites was enhanced for several times on fat; the highest yields of carotenoids (24.8 mg/l) and lipids (54.5%/CDW) were found in S.pararoseus. Simultaneous induction of lipase and biosurfactants was observed on crude fat substrate. Increased C/N ratio (13-100) led to higher biomass production in fat media. Production of total lipids increased in all strains to C/N 50. Oppositely, production of carotenoids, ubiquinone and ergosterol dramatically decreased with increased C/N in all strains. Compounds accumulated in stressed red yeasts are having great application potential and can result from valorization of animal waste fat in the biorefinery concept.
Klíčová slova
carotenogenic yeasts; lipids; carotenoids; lipase; biosurfactants; ubiquinone; ergosterol
Autoři
SZOTKOWSKI, M.; BYRTUSOVÁ, D.; NĚMCOVÁ, A.; VYSOKÁ, M.; RAPTA, M.; SHAPAVAL, V.; MÁROVÁ, I.
Vydáno
19. 11. 2019
Nakladatel
MDPI
ISSN
2076-2607
Periodikum
Microorganisms
Ročník
7
Číslo
11
Stát
Švýcarská konfederace
Strany od
1
Strany do
28
Strany počet
URL
https://www.mdpi.com/2076-2607/7/11/578
Plný text v Digitální knihovně
http://hdl.handle.net/11012/184660
BibTex
@article{BUT160868, author="Martin {Szotkowski} and Dana {Byrtusová} and Andrea {Němcová} and Marie {Vysoká} and Marek {Rapta} and Volha {Shapaval} and Ivana {Márová}", title="Study of Metabolic Adaptation of Red Yeasts to Waste Animal Fat Substrate", journal="Microorganisms", year="2019", volume="7", number="11", pages="1--28", doi="10.3390/microorganisms7110578", issn="2076-2607", url="https://www.mdpi.com/2076-2607/7/11/578" }