Detail publikace

The Quantitative-Phase Dynamics of Apoptosis and Lytic Cell Death

VIČAR, T. RAUDENSKÁ, M. GUMULEC, J. BALVAN, J.

Originální název

The Quantitative-Phase Dynamics of Apoptosis and Lytic Cell Death

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Cell viability and cytotoxicity assays are highly important for drug screening and cytotoxicity tests of antineoplastic or other therapeutic drugs. Even though biochemical-based tests are very helpful to obtain preliminary preview, their results should be confirmed by methods based on direct cell death assessment. In this study, time-dependent changes in quantitative phase-based parameters during cell death were determined and methodology useable for rapid and label-free assessment of direct cell death was introduced. The goal of our study was distinction between apoptosis and primary lytic cell death based on morphologic features. We have distinguished the lytic and non-lytic type of cell death according to their end-point features (Dance of Death typical for apoptosis versus swelling and membrane rupture typical for all kinds of necrosis common for necroptosis, pyroptosis, ferroptosis and accidental cell death). Our method utilizes Quantitative Phase Imaging (QPI) which enables the time-lapse observation of subtle changes in cell mass distribution. According to our results, morphological and dynamical features extracted from QPI micrographs are suitable for cell death detection (76% accuracy in comparison with manual annotation). Furthermore, based on QPI data alone and machine learning, we were able to classify typical dynamical changes of cell morphology during both caspase 3,7-dependent and -independent cell death subroutines. The main parameters used for label-free detection of these cell death modalities were cell density (pg/pixel) and average intensity change of cell pixels further designated as Cell Dynamic Score (CDS). To the best of our knowledge, this is the first study introducing CDS and cell density as a parameter typical for individual cell death subroutines with prediction accuracy 75.4% for caspase 3,7-dependent and -independent cell death.

Klíčová slova

Quantitative phase imaging, cell death, apoptosis, necrosis, cell tracking

Autoři

VIČAR, T.; RAUDENSKÁ, M.; GUMULEC, J.; BALVAN, J.

Vydáno

31. 1. 2020

Nakladatel

Springer Nature

Místo

MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND

ISSN

2045-2322

Periodikum

Scientific Reports

Ročník

10

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

12

Strany počet

12

URL

https://www.nature.com/articles/s41598-020-58474-w

Plný text v Digitální knihovně

http://hdl.handle.net/11012/193482

BibTex

@article{BUT162343,
  author="Tomáš {Vičar} and Martina {Raudenská} and Jaromír {Gumulec} and Jan {Balvan}",
  title="The Quantitative-Phase Dynamics of Apoptosis and Lytic Cell Death",
  journal="Scientific Reports",
  year="2020",
  volume="10",
  number="1",
  pages="1--12",
  doi="10.1038/s41598-020-58474-w",
  issn="2045-2322",
  url="https://www.nature.com/articles/s41598-020-58474-w"
}