Detail publikace

A novel biocompatible titanium–gadolinium quantum dot as a bacterial detecting agent with high antibacterial activity

SUR, V. MAZUMDAR, A. ASHRAFI, A. MUKHERJEE, A. MILOSAVLJEVIĆ, V. MICHÁLKOVÁ, H. KOPEL, P. RICHTERA, L. MOULICK, A.

Originální název

A novel biocompatible titanium–gadolinium quantum dot as a bacterial detecting agent with high antibacterial activity

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this study, the titanium gadolinium quantum dots (TGQDs) were novel, first of its type to be synthesized, and fully characterized to date. Multiple physical characterization includes scanning electron microscopy (SEM), scanning electrochemical microscope (SCEM), x-ray fluorescence, spectrophotometry, and dynamic light scattering were carried out. The obtained results confirmed appropriate size and shape distributions in addition to processing optical features with high quantum yield. The synthesized TGQD was used as a fluorescent dye for bacterial detection and imaging by fluorescent microscopy and spectrophotometry, where TGQD stained only bacterial cells, but not human cells. The significant antibacterial activities of the TGQDs were found against a highly pathogenic bacterium (Staphylococcus aureus) and its antibiotic resistant strains (vancomycin and methicillin resistant Staphylococcus aureus) using growth curve analysis and determination of minimum inhibitory concentration (MIC) analysis. Live/dead cell imaging assay using phase-contrast microscope was performed for further confirmation of the antibacterial activity. Cell wall disruption and release of cell content was observed to be the prime mode of action with the reduction of cellular oxygen demand (OD).

Klíčová slova

bacterial resistance; titanium-gadolinium quantum dots; bacterial detection; antibacterial activity; SECM

Autoři

SUR, V.; MAZUMDAR, A.; ASHRAFI, A.; MUKHERJEE, A.; MILOSAVLJEVIĆ, V.; MICHÁLKOVÁ, H.; KOPEL, P.; RICHTERA, L.; MOULICK, A.

Vydáno

17. 4. 2020

Nakladatel

MDPI

ISSN

2079-4991

Periodikum

Nanomaterials

Ročník

10

Číslo

4

Stát

Švýcarská konfederace

Strany od

1

Strany do

18

Strany počet

18

URL

https://www.mdpi.com/2079-4991/10/4/778

Plný text v Digitální knihovně

http://hdl.handle.net/11012/191379

BibTex

@article{BUT164383,
  author="Vishma Pratap {Sur} and Aninda {Mazumdar} and Amirmansoor {Ashrafi} and Atripan {Mukherjee} and Vedran {Milosavljević} and Hana {Michálková} and Pavel {Kopel} and Lukáš {Richtera} and Amitava {Moulick}",
  title="A novel biocompatible titanium–gadolinium quantum dot as a bacterial detecting agent with high antibacterial activity",
  journal="Nanomaterials",
  year="2020",
  volume="10",
  number="4",
  pages="1--18",
  doi="10.3390/nano10040778",
  issn="2079-4991",
  url="https://www.mdpi.com/2079-4991/10/4/778"
}