Detail publikace

Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk

BEZDĚKOVÁ, J. VLČNOVSKÁ, M. ZEMÁNKOVÁ, K. BAČOVÁ, R. KOLÁČKOVÁ, M. LEDNICKÝ, T. PŘIBYL, J. RICHTERA, L. POMPEIANO VANÍČKOVÁ, L. ADAM, V. VACULOVIČOVÁ, M.

Originální název

Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Dairy cow feed contains, among other ingredients, soybeans, legumes, and clover, plants that are rich in phytoestrogens. Several publications have reported a positive influence of phytoestrogens on human health; however, several unfavorable effects have also been reported. In this work, a simple, selective, and ecofriendly method of phytoestrogen isolation based on the technique of noncovalent molecular imprinting was developed. Genistein was used as a template, and dopamine was chosen as a functional monomer. A layer of molecularly imprinted polymers was created in a microtitration well plate. The binding capability and selective properties of obtained molecularly imprinted polymers were investigated. The imprinted polymers exhibited higher binding affinity toward chosen phytoestrogen than did the nonimprinted polymers. A selectivity factor of 6.94 was calculated, confirming satisfactory selectivity of the polymeric layer. The applicability of the proposed sensing method was tested by isolation of genistein from a real sample of bovine milk and combined with micellar electrokinetic capillary chromatography with UV-visible detection.

Klíčová slova

biochanin A; genistein; dopamine; milk

Autoři

BEZDĚKOVÁ, J.; VLČNOVSKÁ, M.; ZEMÁNKOVÁ, K.; BAČOVÁ, R.; KOLÁČKOVÁ, M.; LEDNICKÝ, T.; PŘIBYL, J.; RICHTERA, L.; POMPEIANO VANÍČKOVÁ, L.; ADAM, V.; VACULOVIČOVÁ, M.

Vydáno

30. 6. 2020

ISSN

0022-0302

Periodikum

JOURNAL OF DAIRY SCIENCE

Ročník

103

Číslo

6

Stát

Spojené státy americké

Strany od

4941

Strany do

4950

Strany počet

10

URL

https://doi.org/10.3168/jds.2019-17367

BibTex

@article{BUT164384,
  author="Jaroslava {Bezděková} and Marcela {Vlčnovská} and Kristýna {Zemánková} and Romana {Bačová} and Martina {Koláčková} and Tomáš {Lednický} and Jan {Přibyl} and Lukáš {Richtera} and Lucie {Pompeiano Vaníčková} and Vojtěch {Adam} and Markéta {Vaculovičová}",
  title="Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk",
  journal="JOURNAL OF DAIRY SCIENCE",
  year="2020",
  volume="103",
  number="6",
  pages="4941--4950",
  doi="10.3168/jds.2019-17367",
  issn="0022-0302",
  url="https://doi.org/10.3168/jds.2019-17367"
}