Detail publikace

Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode

STOJANOVIC, Z. DUROVIC, A. ASHRAFI, A. KOUDELKOVÁ, Z. ZÍTKA, O. RICHTERA, L.

Originální název

Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

A simple and reproducible electroanalytical sensor using antimony trioxide modified-carbon paste electrode (Sb2O3-CPE) as sensing element was employed for the first time for simultaneous determination of glutathione (GSH) and glutathione disulphide (GSSG). The electrochemical response of Sb2O3-CPE was characterized by cyclic voltammetry and square-wave voltammetry. Scanning electron microscopy (SEM), scanning electrochemical microscopy (SECM) and Raman mapping were used to characterize the morphology and surface modification. A significant enhancement in the peak current response of GSH and GSSG accompanied with a good separation of the peaks were obtained at the modified electrode compared to the bare CPE. GSH and GSSG exhibited well-defined oxidation peaks at +1.08 V and +1.36 V (vs. Ag/AgCl, 3 mol/L) in Britton-Robinson buffer at pH 6.0. Parameters such as used supporting electrolyte and its pH value, amplitude and frequency were optimized for square-wave voltammetric determination. Under optimal conditions, voltammetric responses were linear in the concentration range of 2-200 mu mol/L for GSH and GSSG. The limits of detection were 0.34 mu mol/L and 0.10 mu mol/L for GSH and GSSG, respectively. Moreover, the proposed sensor also showed good reproducibility, stability and selectivity. Finally, the proposed sensor was applied for the simultaneous determination of GSH and GSSG in urine samples with satisfactory results.

Klíčová slova

GSH; GSSG; Simultaneous determination; Modified carbon paste electrode; Square-wave voltammetry; Urine

Autoři

STOJANOVIC, Z.; DUROVIC, A.; ASHRAFI, A.; KOUDELKOVÁ, Z.; ZÍTKA, O.; RICHTERA, L.

Vydáno

1. 9. 2020

ISSN

0925-4005

Periodikum

Sensors and Actuators B: Chemical

Ročník

318

Číslo

128141

Stát

Švýcarská konfederace

Strany od

1

Strany do

12

Strany počet

12

URL

https://doi.org/10.1016/j.snb.2020.128141

BibTex

@article{BUT164396,
  author="Zorica {Stojanovic} and Ana {Durovic} and Amirmansoor {Ashrafi} and Zuzana {Koudelková} and Ondřej {Zítka} and Lukáš {Richtera}",
  title="Highly sensitive simultaneous electrochemical determination of reduced and oxidized glutathione in urine samples using antimony trioxide modified carbon paste electrode",
  journal="Sensors and Actuators B: Chemical",
  year="2020",
  volume="318",
  number="128141",
  pages="1--12",
  doi="10.1016/j.snb.2020.128141",
  issn="0925-4005",
  url="https://doi.org/10.1016/j.snb.2020.128141"
}