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Korabecna, M. Zinkova, A. Brynychova, I. Chylikova, B. Prikryl, P. Sedova, L. Neuzil, P. Seda, O.
Originální název
Cell-free DNA in plasma as an essential immune system regulator
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
The cell-free DNA (cfDNA) is always present in plasma, and it is biomarker of growing interest in prenatal diagnostics as well as in oncology and transplantology for therapy efficiency monitoring. But does this cfDNA have a physiological role? Here we show that cfDNA presence and clearance in plasma of healthy individuals plays an indispensable role in immune system regulation. We exposed THP1 cells to healthy individuals’ plasma with (NP) and without (TP) cfDNA. In cells treated with NP, we found elevated expression of genes whose products maintain immune system homeostasis. Exposure of cells to TP triggered an innate immune response (IIR), documented particularly by elevated expression of pro-inflammatory interleukin 8. The results of mass spectrometry showed a higher abundance of proteins associated with IIR activation due to the regulation of complement cascade in cells cultivated with TP. These expression profiles provide evidence that the presence of cfDNA and its clearance in plasma of healthy individuals regulate fundamental mechanisms of the inflammation process and tissue homeostasis. The detailed understanding how neutrophil extracellular traps and their naturally occurring degradation products affect the performance of immune system is of crucial interest for future medical applications.
Klíčová slova
cell free DNA; immune system regulator; cytokine storm
Autoři
Korabecna, M.; Zinkova, A.; Brynychova, I.; Chylikova, B.; Prikryl, P.; Sedova, L.; Neuzil, P.; Seda, O.
Vydáno
1. 12. 2020
Nakladatel
Springer
ISSN
2045-2322
Periodikum
Scientific Reports
Ročník
10
Číslo
1
Stát
Spojené království Velké Británie a Severního Irska
Strany od
17478-1
Strany do
17478-10
Strany počet
URL
https://www.nature.com/articles/s41598-020-74288-2