Detail publikace

Two-dimensional mid and near infrared correlation spectroscopy for bacterial identification

KŘEPELKA, P. PÉREZ-RODRÍGUEZ, F. BOLÍVAR, A.

Originální název

Two-dimensional mid and near infrared correlation spectroscopy for bacterial identification

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In recent years, near infrared (NIR) spectroscopy has gained interest as a tool for bacteria strain identification. Although some promising results suggest good applicability of the technique, a better interpretation of the NIR bacterial spectra is still needed. In order to analyze the NIR spectrum of biological samples, a correlation analysis between the NIR and the mid-infrared (mid-IR) spectra was performed. In total, 28 spectra of 8 bacterial strains were acquired and correlated in the NIR and the mid-IR spectral ranges. Some molecular bands (Amide I, P¼O stretching, C-H stretching/deformation of polysaccharides) were well correlated, and the effect of concentration changes in these molecules were investigated. Moreover, a model for the NIR spectra classification was created with an overall 85% correct classification rate. Subsequently, only NIR wavelengths with high correlation to important mid-IR peaks were selected. This led to an increase in the correct classification rate to 94%. By correlation between well-established mid-IR peaks and NIR spectra, some relationships in the NIR spectra of biological samples were revealed, which was a step towards better understanding and interpretation of the NIR spectra of biological samples.

Klíčová slova

Bacterial identification, classification, near infrared, mid infrared, correlation, 2D correlation, microbiology

Autoři

KŘEPELKA, P.; PÉREZ-RODRÍGUEZ, F.; BOLÍVAR, A.

Vydáno

27. 11. 2020

Nakladatel

SAGE publishing

ISSN

1751-6552

Periodikum

JOURNAL OF NEAR INFRARED SPECTROSCOPY

Ročník

1

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

10

Strany počet

10

URL

https://journals.sagepub.com/doi/10.1177/0967033520974518

BibTex

@article{BUT166451,
  author="Pavel {Křepelka} and Fernando {Pérez-Rodríguez} and Araceli {Bolívar}",
  title="Two-dimensional mid and near infrared correlation spectroscopy for bacterial identification",
  journal="JOURNAL OF NEAR INFRARED SPECTROSCOPY",
  year="2020",
  volume="1",
  number="1",
  pages="1--10",
  doi="10.1177/0967033520974518",
  issn="1751-6552",
  url="https://journals.sagepub.com/doi/10.1177/0967033520974518"
}