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MUSILOVÁ, J. KOUŘILOVÁ, X. BEZDÍČEK, M. LENGEROVÁ, M. OBRUČA, S. ŠKUTKOVÁ, H. SEDLÁŘ, K.
Originální název
First Complete Genome of the Thermophilic Polyhydroxyalkanoates Producing Bacterium Schlegelella thermodepolymerans DSM 15344
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Schlegelella thermodepolymerans is a moderately thermophilic bacterium capable of producing polyhydroxyalkanoates (PHA) – biodegradable polymers representing an alternative to conventional plastics. Here, we present the first complete genome of the type strain S. thermodepolymerans DSM 15344 that was assembled by hybrid approach using both, long (Oxford Nanopore) and short (Illumina) reads. The genome consists of a single 3,858,501 bp long circular chromosome with GC content of 70.3%. Genome annotation identified 3,650 genes in total while 3,598 open reading frames belonged to protein coding genes. Functional annotation of the genome and division of genes into clusters of orthologous groups (COG) revealed a relatively high number of 1,013 genes with unknown function or unknown COG, which reflects the fact that only a little is known about thermophilic PHA producing bacteria on a genome level. On the other hand, 270 genes involved in energy conversion and production were detected. This group covers genes involved in catabolic processes which suggests capability of S. thermodepolymerans DSM 15344 to utilize and biotechnologically convert various substrates such as lignocellulose-based saccharides, glycerol, or lipids. Based on the knowledge of its genome, it can be stated that S. thermodepolymerans DSM 15344 is a very interesting, metabolically versatile bacterium with great biotechnological potential.
Klíčová slova
de novo assembly; hybrid assembly; ONT; functional annotation; PHA
Autoři
MUSILOVÁ, J.; KOUŘILOVÁ, X.; BEZDÍČEK, M.; LENGEROVÁ, M.; OBRUČA, S.; ŠKUTKOVÁ, H.; SEDLÁŘ, K.
Vydáno
12. 1. 2021
Nakladatel
Oxford University Press
ISSN
1759-6653
Periodikum
GENOME BIOL EVOL
Ročník
13
Číslo
4
Stát
Spojené království Velké Británie a Severního Irska
Strany od
1
Strany do
Strany počet
URL
https://academic.oup.com/gbe/article/13/4/evab007/6081016
Plný text v Digitální knihovně
http://hdl.handle.net/11012/196505
BibTex
@article{BUT167680, author="Jana {Musilová} and Xenie {Kouřilová} and Matěj {Bezdíček} and Martina {Lengerová} and Stanislav {Obruča} and Helena {Vítková} and Karel {Sedlář}", title="First Complete Genome of the Thermophilic Polyhydroxyalkanoates Producing Bacterium Schlegelella thermodepolymerans DSM 15344", journal="GENOME BIOL EVOL", year="2021", volume="13", number="4", pages="1--13", doi="10.1093/gbe/evab007", issn="1759-6653", url="https://academic.oup.com/gbe/article/13/4/evab007/6081016" }