Detail publikace

Label-free DNA biosensor using modified reduced graphene oxide platform as a DNA methylation assay

SEDLÁČKOVÁ, E. BYTEŠNÍKOVÁ, Z. BIRGUSOVÁ, E. ŠVEC, P. AMIRMANSOOR, A. ESTRELA, P. RICHTERA, L.

Originální název

Label-free DNA biosensor using modified reduced graphene oxide platform as a DNA methylation assay

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

This work reports the use of modified reduced graphene oxide (rGO) as a platform for a label-free DNA-based electrochemical biosensor as a possible diagnostic tool for a DNA methylation assay. The biosensor sensitivity was enhanced by variously modified rGO. The rGO decorated with three nanoparticles (NPs)-gold (AuNPs), silver (AgNPs), and copper (CuNPs)-was implemented to increase the electrode surface area. Subsequently, the thiolated DNA probe (single-stranded DNA, ssDNA-1) was hybridized with the target DNA sequence (ssDNA-2). After the hybridization, the double-stranded DNA (dsDNA) was methylated by M.SssI methyltransferase (MTase) and then digested via a HpaII endonuclease specific site sequence of CpG (5 '-CCGG-3 ') islands. For monitoring the MTase activity, differential pulse voltammetry (DPV) was used, whereas the best results were obtained by rGO-AuNPs. This assay is rapid, cost-effective, sensitive, selective, highly specific, and displays a low limit of detection (LOD) of 0.06 U center dot mL(-1). Lastly, this study was enriched with the real serum sample, where a 0.19 U center dot mL(-1) LOD was achieved. Moreover, the developed biosensor offers excellent potential in future applications in clinical diagnostics, as this approach can be used in the design of other biosensors.

Klíčová slova

nanomaterials; electrochemical biosensor; DNA methylation; rGO; biomedical applications

Autoři

SEDLÁČKOVÁ, E.; BYTEŠNÍKOVÁ, Z.; BIRGUSOVÁ, E.; ŠVEC, P.; AMIRMANSOOR, A.; ESTRELA, P.; RICHTERA, L.

Vydáno

30. 11. 2020

Nakladatel

MDPI

ISSN

1996-1944

Periodikum

Materials

Ročník

13

Číslo

21

Stát

Švýcarská konfederace

Strany od

1

Strany do

12

Strany počet

12

URL

https://www.mdpi.com/1996-1944/13/21/4936

Plný text v Digitální knihovně

http://hdl.handle.net/11012/195920

BibTex

@article{BUT168307,
  author="Eliška {Dostálová} and Zuzana {Bytešníková} and Eliška {Birgusová} and Pavel {Švec} and Amirmansoor {Ashrafi} and Pedro {Estrela} and Lukáš {Richtera}",
  title="Label-free DNA biosensor using modified reduced graphene oxide platform as a DNA methylation assay",
  journal="Materials",
  year="2020",
  volume="13",
  number="21",
  pages="1--12",
  doi="10.3390/ma13214936",
  issn="1996-1944",
  url="https://www.mdpi.com/1996-1944/13/21/4936"
}