Detail publikace

Characterization and in vitro analysis of probiotic-derived peptides against multi drug resistance bacterial infections

MAZUMDAR, A. HADDAD, Y. SUR, V. MILOSAVLJEVIĆ, V. BHOWMICK, S. MICHÁLKOVÁ, H. GURÁŇ, R. VESELÝ, R. MOULICK, A.

Originální název

Characterization and in vitro analysis of probiotic-derived peptides against multi drug resistance bacterial infections

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

An inexorable switch from antibiotics has become a major desideratum to overcome antibiotic resistance. Bacteriocin fromLactobacillus casei, a cardinal probiotic was used to design novel antibacterial peptides named as Probiotic Bacteriocin Derived and Modified (PBDM) peptides (PBDM1: YKWFAHLIKGLC and PBDM2: YKWFRHLIKKLC). The loop-shaped 3D structure of peptides was characterizedin silicovia molecular dynamics simulation as well as biophysically via spectroscopic methods. Thereafter,in vitroresults against multidrug resistant bacterial strains and hospital samples demonstrated the strong antimicrobial activity of PBDM peptides. Further,in vivostudies with PBDM peptides showed downright recovery of balb/c mice from Vancomycin ResistantStaphylococcus aureus(VRSA) infection to its healthy condition. Thereafter,in vitrostudy with human epithelial cells showed no significant cytotoxic effects with high biocompatibility and good hemocompatibility. In conclusion, PBDM peptides displayed significant antibacterial activity against certain drug resistant bacteria which cause infections in human beings. Future analysis are required to unveil its mechanism of action in order to execute it as an alternative to antibiotics.

Klíčová slova

antibacterial peptides; antibiotics; multidrug resistance; bacteria; infections

Autoři

MAZUMDAR, A.; HADDAD, Y.; SUR, V.; MILOSAVLJEVIĆ, V.; BHOWMICK, S.; MICHÁLKOVÁ, H.; GURÁŇ, R.; VESELÝ, R.; MOULICK, A.

Vydáno

25. 8. 2020

Nakladatel

Frontiers Media

ISSN

1664-302X

Periodikum

Frontiers in Microbiology

Ročník

11

Číslo

1

Stát

Švýcarská konfederace

Strany od

1

Strany do

19

Strany počet

19

URL

https://doi.org/10.3389/fmicb.2020.01963

Plný text v Digitální knihovně

http://hdl.handle.net/11012/196325

BibTex

@article{BUT169138,
  author="Aninda {Mazumdar} and Yazan Abdulmajeed Eyadh {Haddad} and Vishma Pratap {Sur} and Vedran {Milosavljević} and Sukanya {Bhowmick} and Hana {Michálková} and Roman {Guráň} and Radek {Veselý} and Amitava {Moulick}",
  title="Characterization and in vitro analysis of probiotic-derived peptides against multi drug resistance bacterial infections",
  journal="Frontiers in Microbiology",
  year="2020",
  volume="11",
  number="1",
  pages="1--19",
  doi="10.3389/fmicb.2020.01963",
  issn="1664-302X",
  url="https://doi.org/10.3389/fmicb.2020.01963"
}