Detail publikace

Computational design of enzymes for biotechnological applications

PLANAS-IGLESIAS, J. PINTO, G. MARQUES, S. MUSIL, M. DAMBORSKÝ, J. ŠTOURAČ, J. BEDNÁŘ, D.

Originální název

Computational design of enzymes for biotechnological applications

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Enzymes are the natural catalysts that execute biochemical reactions upholding life. Their natural effectiveness has been fine-tuned as a result of millions of years of natural evolution. Such catalytic effectiveness has prompted the use of biocatalysts from multiple sources on different applications, including the industrial production of goods (food and beverages, detergents, textile, and pharmaceutics), environmental protection, and biomedical applications. Natural enzymes often need to be improved by protein engineering to optimize their function in non-native environments. Recent technological advances have greatly facilitated this process by providing the experimental approaches of directed evolution or by enabling computer-assisted applications. Directed evolution mimics the natural selection process in a highly accelerated fashion at the expense of arduous laboratory work and economic resources. Theoretical methods provide predictions and represent an attractive complement to such experiments by waiving their inherent costs. Computational techniques can be used to engineer enzymatic reactivity, substrate specificity and ligand binding, access pathways and ligand transport, and global properties like protein stability, solubility, and flexibility. Theoretical approaches can also identify hotspots on the protein sequence for mutagenesis and predict suitable alternatives for selected positions with expected outcomes. This review covers the latest advances in computational methods for enzyme engineering and presents many successful case studies.

Klíčová slova

biocatalyst,catalytic efficiency,computational enzyme design,enzyme,biotechnologies,protein engineering,protein dynamics,software,solubility,stability

Autoři

PLANAS-IGLESIAS, J.; PINTO, G.; MARQUES, S.; MUSIL, M.; DAMBORSKÝ, J.; ŠTOURAČ, J.; BEDNÁŘ, D.

Vydáno

18. 2. 2021

ISSN

0734-9750

Periodikum

BIOTECHNOLOGY ADVANCES

Ročník

47

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

22

Strany počet

22

URL

https://doi.org/10.1016/j.biotechadv.2021.107696

BibTex

@article{BUT169805,
  author="PLANAS-IGLESIAS, J. and PINTO, G. and MARQUES, S. and MUSIL, M. and DAMBORSKÝ, J. and ŠTOURAČ, J. and BEDNÁŘ, D.",
  title="Computational design of enzymes for biotechnological applications",
  journal="BIOTECHNOLOGY ADVANCES",
  year="2021",
  volume="47",
  number="1",
  pages="1--22",
  doi="10.1016/j.biotechadv.2021.107696",
  issn="0734-9750",
  url="https://doi.org/10.1016/j.biotechadv.2021.107696"
}