Detail publikace

Image-Based Motion Correction for Optical Mapping of Cardiac Electrical Activity

KHWAOUNJOO, P. RUTHERFORD, S. ŠVRČEK, M. LEGRICE, I. TREW, M. SMAILL, B.

Originální název

Image-Based Motion Correction for Optical Mapping of Cardiac Electrical Activity

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Optical mapping, with membrane-bound, voltage-sensitive dyes, is widely used for in vitro recording of cardiac electrical activity. The spatial registration of such maps is lost when the heart moves with respect to a fixed photodetector array and contraction can generate substantial artifact if background fluorescence is not uniformly distributed. While motion artifact is commonly suppressed with electromechanical uncoupling agents, there are circumstances where these are undesirable. This study outlines a novel image-based approach for retrospective motion artifact correction. Isolated Langendorff-supported rat hearts (n = 8), stained with di-4-ANEPPS, were illuminated at 516 +/- A 14 nm and fluorescent emission (> 565 +/- A 10 nm) was acquired with a charge multiplying CCD camera. Background fluorescence was segmented in successive frames and stabilized using a non-rigid image registration algorithm. The resultant image deformation was used to estimate material point movement on the heart surface, so that total fluorescence could be mapped frame-by-frame to appropriate reference pixels. Finally, residual motion artifact was identified and removed. The effectiveness of this correction method was evaluated over 18 experimental datasets. Signal-to-noise ratio was increased more than fourfold, and activation time and action potential duration (APD) could be estimated at 24% more pixels than in the raw data. The variability of all APD measures was substantially reduced (i.e. APD50 estimated as 83.8 +/- A 45.8 ms before correction was 52.1 +/- A 4.7 ms afterward). This approach provides a robust means of recovering optical action potentials in the presence of substantial motion artifact.

Klíčová slova

Optical mapping; Cardiac; Electrical activation; Motion correction; Active demons algorithm; Electrophysiology

Autoři

KHWAOUNJOO, P.; RUTHERFORD, S.; ŠVRČEK, M.; LEGRICE, I.; TREW, M.; SMAILL, B.

Vydáno

11. 11. 2014

Nakladatel

SPRINGER

Místo

NEW YORK

ISSN

1573-9686

Periodikum

ANNALS OF BIOMEDICAL ENGINEERING

Ročník

43

Číslo

5

Stát

Spojené státy americké

Strany od

1235

Strany do

1246

Strany počet

12

URL

https://link.springer.com/article/10.1007%2Fs10439-014-1172-8

BibTex

@article{BUT169896,
  author="Prashanna {Khwaounjoo} and Sally {Rutherford} and Martin {Švrček} and Ian {LeGrice} and Mark {Trew} and Bruce {Smaill}",
  title="Image-Based Motion Correction for Optical Mapping of Cardiac Electrical Activity",
  journal="ANNALS OF BIOMEDICAL ENGINEERING",
  year="2014",
  volume="43",
  number="5",
  pages="1235--1246",
  doi="10.1007/s10439-014-1172-8",
  issn="1573-9686",
  url="https://link.springer.com/article/10.1007%2Fs10439-014-1172-8"
}