Detail publikace

LC-MS/MS identification of proteins from a porcine lung tissue affected by Actinobacillus pleuropneumoniae

DO, T. JAROŠOVÁ, R. SEDLÁČKOVÁ, E. GURÁŇ, R. ONDRÁČKOVÁ, P. FALDYNA, M. SLÁDEK, Z. ZÍTKA, O.

Originální název

LC-MS/MS identification of proteins from a porcine lung tissue affected by Actinobacillus pleuropneumoniae

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

Targeted therapy of porcine pleuropneumonia, which is caused by the bacterium Actinobacillus pleuropneumoniae, would help pig breeders to lower their expenses related with solving this problem. In order to find potential receptors of a targeted therapy it is important to know the response of the organismon an infection and detect proteins connected to an immune reaction. Nowadays, a liquid chromatography connected with tandem mass spectrometry (LC-MS/MS) is one of the most used methods for this purpose. This study was aimed at detection and identification of proteins after homogenization and extraction from the porcine lung tissue cryosections by applying LC-MS/MS method using high-performance liquid chromatography-electrospray ionisation quadrupole-quadrupole time-of-flight mass spectrometry (HPLC-ESI-QqTOF MS).

Klíčová slova

pig; liquid chromatography; tandem mass spectrometry; electrospray-time of flight mass spectrometry; pulmonary infection; proteins

Autoři

DO, T.; JAROŠOVÁ, R.; SEDLÁČKOVÁ, E.; GURÁŇ, R.; ONDRÁČKOVÁ, P.; FALDYNA, M.; SLÁDEK, Z.; ZÍTKA, O.

Vydáno

6. 11. 2019

ISBN

978-80-7509-688-3

Kniha

MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE

Strany od

563

Strany do

568

Strany počet

6

BibTex

@inproceedings{BUT170318,
  author="Tomáš {Do} and Rea {Jarošová} and Eliška {Dostálová} and Roman {Guráň} and Petra {Ondráčková} and Martin {Faldyna} and Zbyšek {Sládek} and Ondřej {Zítka}",
  title="LC-MS/MS identification of proteins from a porcine lung tissue affected by Actinobacillus pleuropneumoniae",
  booktitle="MENDELNET 2019: PROCEEDINGS OF 26TH INTERNATIONAL PHD STUDENTS CONFERENCE",
  year="2019",
  pages="563--568",
  isbn="978-80-7509-688-3"
}